Crepidula fornicata Developmental Transcriptome Data
Sequence information is also available through NCBIat dbEST and the Trace Archives.
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RNA was collected from Crepidula fornicata during the first five days of development and subjected to 454 pyrosequencing. The single batch plate was split into two files retrievable here. The three titration runs conducted to determine parameters for the batch plate are also provided here. |
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Paired-end, stranded 250bp reads assembled using TrinityRNASeq v2.2.0 running default settings, including defaults for read trimming (Trimmomatic as implemented in TrinityRNASeq) and normalization (as implemented in Trinity RNASeq). Assembled using kmer size of 25bp (default) and 31bp.
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The results of 454 pyrosequencing were assembled into contigs and are available here. *
The various parameters used to assemble the contigs are provided in this Seqman Report. |
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The sequences that could not be aligned to build the contigs are available here. |
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The Crepidula fornicata 454 annotations compiled show the top blast hits for each contig. |
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This research is funded by N.S.F. Grant IOS 1558061 to J.J.H.