Arabidopsis Calmodulin Expression Vectors
Calmodulin (CaM) is easily expressed and purified from cultures of transformed E. coli. The table below lists the different types of CaM-expressing plasmids we have constructed. All are based on the pET vector backbones. They all are stable and can be expressed in E. coli BL21(DE3). For mutagenesis and routine cloning, however, an E. coli K12 strain should be used like TOP10, XL10 or DH5a.
| Plasmid | Expressed Protein |
Parent Vector
|
Selectable Marker
|
Sequence
|
Plasmid Map
|
| pRZ16 | unfused CaM2 (same as CaM3 and 5) |
pET5a
|
ampicillin
|
pRZ16.txt
|
pRZ16.pdf |
| pRZ41 | unfused CaM4 (same as CaM1) |
"
|
"
|
pRZ41.txt
|
pRZ41.pdf |
| pRZ61 | unfused CaM6 |
"
|
"
|
pRZ61.txt
|
pRZ61.pdf |
| pRZ72 | unfused CaM7 |
pET24d
|
kanamycin
|
pRZ72.txt
|
pRZ72.pdf |
| pRZ82 | unfused CML8 (aka CaM8) |
"
|
"
|
pRZ82.txt
|
pRZ82.pdf |
| pRZ95 | unfused CML9 (aka CaM9) |
"
|
"
|
pRZ95.txt
|
pRZ95.pdf |
| pRZ95S | 6X His:SUMO:CML9 |
pE-SUMO
|
"
|
pRZ95s.txt
|
pRZ95s.pdf |
| pRZ108 | 6XHis:GFP:CaM2 |
pET24d
|
"
|
pRZ108a.txt
|
pRZ108a.pdf |
| pRZ109 | 6X His:BFP:CaM2 |
"
|
"
|
pRZ109a.txt
|
pRZ109a.pdf |
| pRZ496 | 6X His:GFP:CML9 |
"
|
"
|
pRZ496.txt
|
pRZ496.pdf |
| pRZ497 | 6X His:CML9 |
"
|
"
|
pRZ497.txt
|
pRZ497.pdf |
| pRZ514c | 6X His:CFP:CML9 |
"
|
"
|
pRZ514c.txt
|
pRZ514c.pdf |
| pRZ514y | 6X His:YFP:CML9 |
"
|
"
|
pRZ514y.txt
|
pRZ514y.pdf |
| pRZ515c | 6X His:CFP:CML8 |
"
|
"
|
pRZ515c.txt
|
pRZ515c.pdf |
| pRZ515y | 6X His:YFP:CML8 |
"
|
"
|
pRZ515y.txt
|
pRZ515y.pdf |
| pRZ516c | 6X His:CFP:CaM5 |
"
|
"
|
pRZ516c.txt
|
pRZ516c.pdf |
| pRZ516y | 6X His:YFP:CaM5 |
"
|
"
|
pRZ516y.txt
|
pRZ516y.pdf |
| pRZ517 | CaM5:YFP:8X His |
"
|
"
|
pRZ517.txt
|
pRZ517.pdf |
| pRZ654 | unfused CaM5 (same as CaM2 and 3) |
"
|
"
|
pRZ654.txt
|
pRZ654.pdf |
| pRZ655 | 6X His:CaM5 |
"
|
"
|
pRZ655.txt
|
pRZ655.pdf |
| pRZ656 | 6X His:CaM4 |
"
|
"
|
pRZ656.txt
|
pRZ656.pdf |
| pRZ657 | 6X His:CaM6 |
"
|
"
|
pRZ657.txt
|
pRZ657.pdf |
| pRZ658 | 6X His:CaM7 |
"
|
"
|
pRZ658.txt
|
pRZ658.pdf |
| pRZ659 | 6X His:CML8 |
"
|
"
|
pRZ659.txt
|
pRZ659.pdf |
| pRZ660 | unfused CML11 |
"
|
"
|
pRZ660.txt
|
pRZ660.pdf |
| pRZ661 | 6X His:CML11 |
"
|
"
|
pRZ661.txt
|
pRZ661.pdf |
Our protocol for purifying recombinant CaM from E. coli can be downloaded through this link.
This page is maintained by Ray Zielinski and was last updated in 13 August 2010.