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[microwave] After the agarose has been weighed and the buffer measured, the two are mixed. But, as mentioned before, since agarose is not soluble in buffer at room temperature (based on what you know at this point, can you figure out why?), it must be boiled. The fastest way to do this is by putting the flask in the microwave, as shown in the photo. The volume of buffer in the photo probably came to a boil in just about one minute.


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