FastQCFastQC Report
Tue 18 Oct 2016
C_atra_RNA1_ATCACG_L002_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameC_atra_RNA1_ATCACG_L002_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169567079
Sequences flagged as poor quality0
Sequence length35-250
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAATCATAACAATATATAATCAATTAAAACATTTAAAAATTTAGTAAA7176140.4232036101771854No Hit
GTTTATCACAATCATAACAATATATAATCAATTAAAACATTTAAAAATTT4729380.2789090917819018No Hit
ATCCATTTCACCTAGTAGAGTTTAGCCCTTGACCCTTAACAGGTTCAATA3899520.22996916754106497No Hit
ATTTTTTCTTCGTTCGACGATAATAACCAAGTTTTTATGTCTTCATACTT3419100.2016370170532925No Hit
CACAATCATAACAATATAATCAATTAAAACATTTAAAAATTTAGTAAAGG3391070.19998398391942576No Hit
CTTTTTTCAACAAACCATAAAGATATTGGTACTTTATATATTTTATTCGG3320220.195805696458332No Hit
GGACAATTAGGATTCCAGGATGCAGCTTCTCCTTTAATGGAAGAGCTAAT2322900.13699003448658806No Hit
GTTTATCACAATCATAACAATATAATCAATTAAAACATTTAAAAATTTAG2276500.13425365427212438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTAG1654150.077.266449
TCATAAC4258500.062.750056
CACAATC4515450.060.0842821
TCACCTA2289750.056.332658
TTATCAC3288500.054.164193
GTTTATC3450850.052.7903141
CATAACA5112500.052.593657
CAATTAG1461850.051.007694
CAATCAT5266950.051.001793
ATCATAA5280700.050.8040055
TATCACA3544650.050.3303684
AATCATA5406050.049.6628534
ACAATCA5993550.044.861462
TTCGTTC2221750.039.9825829
ATCCATT3279800.039.9437451
TTCACCT3394000.039.2506757
TTCTTCG2357100.038.5549966
TAACAAT7365750.037.041819
CTATTCG892350.035.2551772
CCATTTC3851250.034.813393