FastQCFastQC Report
Tue 18 Oct 2016
C_atra_RNA1_ATCACG_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameC_atra_RNA1_ATCACG_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169567079
Sequences flagged as poor quality0
Sequence length35-250
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTATACATTTTAAGGAAAACTAGCTATCATTTGATTCGGAAAAAATA3367590.1985992811729687No Hit
GTCCTTTCGTACTAAATTTATTATTTGAATTAAAGATAGAAACTGACCTG2815550.16604343346623313No Hit
GTTCCTTAGTCGCCCCAACCAAAATTTTTAATAATTTTTTTTTACAAGTA1714030.10108271075424966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA1057650.068.325758
CCTTTCG1425000.051.4496353
TTCGTAC1652650.044.941816
TTTCGTA1920800.038.2263455
TCGTACT2145000.034.6964347
CTTTCGT2190150.033.8299144
CGTCTAC1074650.032.270333
GTCCTTT3067600.031.6131341
CTTCGAT1149450.030.2557851
TCTATAC2955150.028.7321683
GTCTACG1180100.028.450744
TCGATTC1144750.027.452483
CTACGAA1349600.025.3550171
TACGAAT1350700.024.998177
GTACTAA3128300.023.2904289
CGATTCA1629500.021.870664
CAGAACG1424150.020.8798759
CTATACA4372550.020.1123964
TCTACGA1681500.019.8228025
GATTCAG1787050.019.385575
Produced by FastQC (version 0.11.5)