Ed Berry's structure of the bc1-complex (Chicken heart mitochondria) |
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Ed Berry's structure of the bc1-complex (Chicken heart mitochondria) |
Spectra and Crystals of the bc1-complex from Ed Berry, and the Q-cycle mechanism.
The annual synthesis (and consumption) of biomass in the biosphere represents a storage of energy 20-100 fold greater than all human energy usage, including fossil fuels and nuclear power. Because of inefficiencies in energy conversion, the flux in the biosphere is about 1000-fold greater than that through all anthropogenic processes.
In the interests of rapid dissemination of research materials within the group, some of the material accessible through this site is made available before publication. It is expected that any use of such material will be appropriately acknowledged.
Follow the links below to find additional information.
In the Chime tutorials (below), features of interest are accessible through buttons. Tutorials are available for the 3-subunit "functional" monomer, and also for the dimeric complexes. The former can be used to explore features of mechanistic interest.
The third link below is to a "working model" showing a speculative mechanism from the Crofts group, demonstrated through a Chime animation. The model is heavily annotated, with justification for the different assumptions underlying the mechanism, together with references to the literature. The model is based on the Zhang et al. structures, but incorporates different configurations of the ISP from both the Zhang et al. and Iwata et al. coordinates, and additional models (quinone species at the Qo-site, electrons and protons) necessary for the animation.
Mechanism in an annotated animated GIF (~4 MB, but runs as it loads)
Mechanism in an annoted AVI movie (~6.7 MB)
Chime
here.A brief summary
of the files (1bcc.pdb, 1bccdimref.pdb, 1bcc_m.pdb, 3bcc.pdb, 3bccdimref.pdb,
3bcc_m.pdb) is available here (see also 1bcc3bcc.txt).
The Iwata et al. coordinates for the beef heart mitochondrial bc1
complex
The files are p65.pdb and p6522.pdb
Les Dutton's cartoons of the UPenn Biochemistry and Biophysics faculty.
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