Known and Putative CaM-Regulated Proteins in Plants

          The table below lists the known and suspected CaM-regulated proteins in Arabidopsis and gives the criteria by which they were identified.  This list was compiled from several references, given at the bottom of this page.  Hyperlinks to these articles are given.

 

          Direct screening of cDNA expression libraries for CaM-binding proteins (CaM-BPs) has been carried out using CaM labeled by a variety of methods, including: biotinylation (Bio-CaM), conjugation with alkaline phosphatase (AP-CaM), or radiolabeling in vivo with 35S-methionine (35S-CaM).  By identifying the functions of CaM-BPs proteins by cloning, we will be able to 1) identify key processes in plants that are subject to second messenger Ca2+ signaling; 2) biochemically define the interaction between plant calmodulin and its targets; and 3) use the cDNA clones as in situ hybridization tools to localize the cell types in plants that might critically respond to Ca2+ signaling. Our current working hypothesis is that the plant epidermis will be an important region in signaling environmental stimuli, and the cDNA clones isolated in this work will allow us to test this idea and delineate the role of epidermal cells in processing environmental signals.

 

Protein

Gene ID

Amino acids

Method of

Identification

CaM-binding

sequence

 1. Hypothetical

At5g04020

1495

E

 

 2. PKC substrate-like

At5g56360

647

E

 

 3. Photosystem I-N

At5g64040

171

E, BH

 

 4. Hypothetical

At2g38800

612

E, BH

 

 5. KCBP

At5g65930

1260

E

 

 6. ATPase

At3g56690

1022

E

 

 7. Chaperonin 10g

At5g20720

253

E

 

 8. Glyoxalase

At1g08110

196

PH

 

 9. Apyrase

At3g04080

471

PH

 

10. Multidrug-resistant-like

At2g36910

1286

PH

 

11. Ser/Thr phosphatase PP7

At5g63870

413

PH

 

12. SAURs

 

 

 

 

  SAUR.a

At3g43120

160

E, BH

 

  SAUR.b

At5g20810

190

PH

 

  SAUR.c

At4g31320

189

PH

 

  SAUR.d

At2g24400

178

PH

 

  SAUR.e

At4g34750

150

PH

 

  13. Hypothetical.a

At3g25030

250

E, BH

 

  14. Hypothetical.b

At4g13100

304

PH

 

  ACBP60-like.a

At5g62570

505

E

 

  ACBP60-like.b

At5g57580

647

E

 

  ACBP60-like.c

At2g18750

652

E

 

  ACBP60-like.d

At4g25800

536

E

 

  ACBP60-like.e

At2g24300

503

E, BH

 

  ACBP60-like.f

At4g31000

467

PH

 

  ACBP60-like.g

At5g26920

492

PH

 

15. CNGCs

 

 

 

 

  CNGC1

At5g53130

716

E, BH

 

  CNGC2

At5g15410

726

E

 

  CNGC3

At2g46430

718

E

 

  CNGC4

At5g54250

694

E

 

  CNGC5

At5g57940

710

E

 

  CNGC6

At2g23980

688

E, BH

 

  CNGC7

At1g15990

709

PH

 

  CNGC8

At2g19780

728

PH

 

  CNGC9

At2g30560

733

PH

 

  CNGC10

At2g01340

711

PH

 

  CNGC11

At3g46440

 

PH

 

  CNGC12

At4g46450

 

PH

 

  CNGC13

At4g01010

 

PH

 

  CNGC14

At4g24610

 

E, BH

 

  CNGC15

At5g28260

 

PH

 

  CNGC16

At1g48010

 

PH

 

  CNGC17

At1g30360

 

PH

 

  CNGC18

At2g14870

 

PH

 

  CNGC19

At3g17690

743

PH

 

  CNGC20

At3g17700

773

PH

 

16. EICBPs

 

 

 

 

  EICBP.a

At2g22300

1042

E

 

  EICBP.b

At5g09410

1007

E

 

  EICBP.c

At5g64220

1014

PH

 

  EICBP.d

At1g67310

1016

PH

 

  EICBP.e

At3g16940

838

PH

 

  EICBP.f

At4g16150

954

PH

 

17. Pirin-like proteins

 

 

 

 

  Pirin-like.a

At2g43120

270

E

 

  Pirin-like.b

At3g59220

287

PH

 

  Pirin-like.c

At3g59260

271

PH

 

  Pirin-like.d

At1g50590

 

 

 

 

 

 

 

a  The protein names shown in bold were isolated in our screening (clones 1, 2, and 5 are from Arabidopsis and 3 and 4 are Arabidopsis homologs of bean CBPs. The KCBP (20), ATPase (86), chaperonin 10 (87), and Ser/Thr phosphatase (88) were isolated from Arabidopsis previously. Homologs to glyoxalase (89), apyrase (90), and multidrug resistance proteins (91) were isolated from other plants.

b  Arabidopsis cDNA libraries used in screening were prepared from flower meristem (FM) or tissues treated with auxin (AuxT), elicitor (ET) or ethylene (EtT).

c  The CBP was isolated by screening expression libraries with labeled CaM (E) or identified based on sequence similarity to bean clones (BH) or known plant (PH) CBP.