David Lampe

Current address:

Department of Biological Sciences

Mellon Hall

Bayer School of Natural and Environmental Sciences

Duquesne University

Pittsburg

PA 15282-1502

Phone: 412-396-6322

FAX: 412-396-5907

Email: lampe@duq.edu


Update

David moved to the Department of Biological Sciences at Duquesne University in Pittsburg in the Fall of 1998 as an assistant professor, tenured in 2004, and continues to work on mariner transposons, specifically the biochemistry of a particular version called Himar1 that he developed while working at Illinois as a postdoc, and development of it as a tool for diverse insects and other organisms. He is now an Associate Professor there.

As a postdoc working with me from 1992-1998, David achieved the major breakthroughs in the work towards developing active versions of mariners that might serve as genetic tools. Thus we isolated and sequenced multiple clones of irritans and mellifera subfamily mariners, and developed the Himar1 element from the hornfly Haematobia irritans, which has already proven successful in bacteria, Drosophila, and human cells.


Papers derived from David's work with us are:

Robertson, H. M., D. J. Lampe and E. G. MacLeod 1992. A mariner transposable element from a lacewing. Nucleic Acids Research. 20, 6409.


Robertson, H. M. and D. J. Lampe 1995a. Recent horizontal transfer of a mariner element between Diptera and Neuroptera. Mol. Biol. Evol. 12, 850-862.

Robertson, H. M. and D. J. Lampe 1995b. Distribution of transposable elements in arthropods. Ann. Rev. Entomol. 40, 333-357.

Lampe, D. J., M. E. A. Churchill and H. M. Robertson. 1996. A purified mariner transposase is sufficient to mediate transposition in vitro. EMBO J. 15, 5470-5479.

Chen, C-L., D. J. Lampe, H. M. Robertson and J. B. Nardi 1997. Neuroglian is expressed by cells destined to form the prothoracic glands of Manduca embryos as they segregate from surrounding cells and rearrange during morphogenesis. Developmental Biology 181, 1-13.

Lampe, D. J., T. E. Grant and H. M. Robertson 1998. Factors affecting transposition of the Himar1 mariner transposon in vitro. Genetics 149, 179-187.

Robertson, H. M., F. N. Soto-Adames, K. K. O. Walden, R. M. P. Avancini, and D. J. Lampe. 1998. The mariner transposons of animals: horizontally jumping genes. In: Horizontal Gene Transfer. Ed. M. Syvanen and C. Kado. pp 268-284. Chapman and Hall, London.

Akerley, B. J., E. J. Rubin, A. Camilli, D. J. Lampe, H. M. Robertson and J. J. Mekalanos 1998. Systematic identification of essential genes by in vitro mariner mutagenesis. Proc. Natl. Acad. Sci. USA 95, 8927-8932.

Zhang, L. U. Sankar, D. J. Lampe, H. M. Robertson, and F. L. Graham 1998. The Himar1 mariner transposon cloned in a recombinant adenovirus vector is functional in mammalian cells. Nucl. Acids Res. 26, 3687-3692.

Lampe, D. J., Ackerley, B. J., Rubin, E. J., Mekalanos, J. J. and H. M. Robertson. 1999. Hyperactive transposase mutants of the Himar1 mariner transposon. Proceedings of the National Academy of Science USA 96, 11428-11433.

Nardi, J. B., R. Martos, K. K. O. Walden, D. J. Lampe and H. M. Robertson. 1999. Expression of lacunin, a large multidomain extracellular matrix protein, accompanies morphogenesis of epithelial monolayers in Manduca sexta. Insect Biochemistry and Molecular Biology 29, 883-897.

Lampe, D. J., K. K. O. Walden, J. M. Sherwood, and H. M. Robertson 2000. Genetic engineering of insects with mariner transposons. In: Insect Transgenesis: Methods and Applications. Ed. A. Handler and T. James. pp237-248. CRC Press.

Zhang, J. K., M. A. Pritchett, D. J. Lampe, H. M. Robertson and W. W. Metcalf. 2000. In vivo transposon mutagenesis of the methanogenic archaeon Methanosarcina acetivorans C2A using a modified version of the insect mariner-family transposable element Himar1. Proceedings of the National Academy of Sciences USA 97, 9665-9970.

Lampe, D. J., K. K. O. Walden, and H. M. Robertson. 2001. Loss of transposase-DNA interaction may underlie the divergence of marinerfamily transposable element and the ability of more than one mariner to occupy the same genome. Molecular Biology and Evolution 18, 954-961.

Lampe, D. J., D. J. Witherspoon, F. N. Soto-Adames, and H. M. Robertson. 2003. Recent horizontal transfer of mellifera subfamily mariner transposons into insect lineages representing four different orders shows that selection acts only during horizontal transfer. Molecular Biology and Evolution 20, 554-562.



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