Carol Shearer

 

Emeritus Professor of Plant Biology

655 Morrill MC-116
(217) 333-2796

Education

Ph.D., 1971, University of Maryland

Research

In freshwater habitats, leaves and branches of riparian vegetation are important as a food resource for stream heterotrophs. Fungi serve as intermediaries in making this resource more palatable and nutritious for aquatic invertebrates. We are interested in finding out why certain fungal species are more successful in colonizing resources initially, why other species are able to displace initial colonists over time, and how environmental factors influence colonization and decomposition processes. Field and laboratory studies are used to determine the relative importance of intrinsic characteristics of species that comprise their overall ability to colonize and decompose resources. These characteristics include: spore germination and germ tube elongation rates, growth rates, enzymatic capabilities, and ability to produce and resist antibiotics. Interspecific interactions among fungal species and between fungi and other organisms, such as grazing invertebrates, are also important determinants of species success in resource colonization. Field and laboratory experiments in which the interacting species are manipulated are used to determine the effects of species interactions on colonization processes.


Systematic studies in my laboratory deal with two general areas: fungal geography and biodiversity and phylogenetic relationships in Ascomycetes. The number of fungal species has been conservatively estimated at 1.5 million but only about 69,000 species actually have been collected and described. The freshwater ascomycetes, a group that has only recently been studied, are very poorly known. As part of the effort to determine fungal biodiversity as it relates to latitude, we are collecting fresh-water ascomycetes and their asexual states along latitudinal gradients through North America, which encompass arctic through tropical biomes. Species are collected, identified, described if new or rare, isolated in pure culture, and tested for the production of bioactive compounds.


Phylogenetic relationships among ascomycete taxa are poorly understood. This is especially true for aquatic and marine species which have undergone convergent evolution in response to habitat. We use cladistic analyses of morphological and molecular sequence data to resolve relationships among morphologically similar freshwater, marine and terrestrial species.


Publications

Oh, H., D.C. Swenson, J.B. Gloer and C.A. Shearer. 2003. New bioactive rosigenin analogues and aromatic polyketide metabolites from the freshwater aquatic fungus Massarina tunicata. J. Nat. Prod. 66: 73–79.

 

Campbell, J., C.A. Shearer, J.L. Crane, and P. Fallah. 2003. A reassessment of two freshwater ascomycetes, Ceriospora caudae-suis and Submersisphaeria aquatica.  Mycologia 95: 41–53.

 

Schmit, J.P. and C.A. Shearer. 2003. A checklist of mangrove-associated fungi, their geographical distribution and known host plants. Mycotaxon 85: 423–477.

 

Campbell, J., J. Anderson, and C.A. Shearer. 2003. Systematics of Halosarpheia based on morphological and molecular data. Mycologia: 95:532–553

 

Raja, H. A., J. Campbell, and C.A. Shearer. 2003. Freshwater ascomycetes:  Cyanoannulus petersenii, a new genus and species from submerged wood. Mycotaxon 78: 1–17.

 

Li C., Nitka M, Gloer J.B., Campbell J. and Shearer C.A. 2003. Annularins A-H: new polyketide metabolites from the freshwater fungus Annulatascus triseptatus. J. Nat. Prod. 66: 1302–1306.

 

Shearer, C.A., Deborah M. Langsam and Joyce E. Longcore. 2004. Fungi in Freshwater Habitats. Chapter 23 In: Measuring and Monitoring Biological Diversity: Standard Methods for Fungi. Mueller, G. M., G. F. Bills, and M. S. Foster, Eds. Smithsonian Institution Press, Washington, D.C. 777 pp.

 

Campbell, J. and C.A. Shearer. 2004. Annulusmagnus and Ascitendum, two new genera in the Annulatascaceae. Mycologia 96: 821-832. Mycologia 96: 821– 832.

 

Schmit, J.P., and C.A. Shearer. 2004. Geographic and host distribution of lignicolous mangrove microfungi. Botanica Marina 47: 496–500.

 

Reátegui R, Gloer JB, Campbell J and Shearer CA. 2005. Ophiocerins A-D: New Tetrahydropyran derivatives from the freshwater aquatic fungus Ophioceras venezuelense. J. Nat. Prod. 68: 701–705.

 

Sina M. Adl, Alastair G.B. Simpson, Mark A. farmer, Robert A.  Andersen, Roger A. Anderson, John Barta, Samuel Bowser, Guy Brugerolle, Robert Fensome, Suzanne Fredericq, Timothy Y. James, Sergei Karpov, Paul Kugrens, John Krug, Chris Lane, Louise A. Lewis, Jean Lodge, Denis H. Lynn, David Mann, Richard M. McCourt, Leonel Mendoza, Ojvind Moestrup, Sharon E. Mozeley-Standridge, Thomas A. Nerad, Carol Shearer, Frederick Spiegel, Max F.J.R. Taylor. 2005. The new higher level classification of eukaryotes with emphasis on the taxonomy of protists. Journal of Eukaryotic Microbiology 52(5): 399–451.

 

Raja, H., A. Ferrer, and C.A. Shearer. 2005. Aliquandostipite crystallinum, a new species from submerged wood in freshwater habitats. Mycotaxon: 207–215.

 

Ferrer, A. and C.A. Shearer. 2005. New records and a new species of Canalisporium from aquatic habitats in Panama. Mycotaxon 93: 179–188.

 

Campbell, J., C. A. Shearer and L. Marvanová. 2006. Evolutionary relationships among aquatic anamorphs and teleomorphs: Lemonniera, Margaritispora, Goniopila. Mycol. Res. 110(9):  1025–1033.

 

Anderson, J.L., J. Beever, and C.A. Shearer. 2006. Eight polymorphic microsatellite loci for the aquatic fungus Tetracladium marchalianum. Molecular Ecology Notes 6(3): 703–706.

 

Raja, H. A. and C. A. Shearer. 2006. Arnium gigantosporum, a new ascomycete species from fresh water in Florida. Fungal Diversity 22: 219–225.

 

Miller, A. N., C. A. Shearer, M. Bartolata and S. M. Huhndorf. 2006. Cuspidatispora xiphiago gen. et sp. nov. from an eastern North American creek. Mycoscience 47: 218–233.

 

Sanjay V. Mudur, Dale C. Swenson, James B. Gloer, Jinx Campbell, and Carol A. Shearer. 2006. Heliconols A-C:  Polyketide-derived Hemiketals from the Freshwater Aquatic Fungus Helicodendron giganteum Glen-Bott (Helotiaceae) Organic Letters 8 (15): 3191–3194.

 

Raja, H.A. and C.A. Shearer. 2006. Jahnula species from North and Central America, including three new species. Mycologia 98: 319–332.

 

Raja, H.A., A.M. Stchigel, A.N. Miller, J.L. Crane, C.A. Shearer, and J. Guarro. 2006. Mitosporic euascomycete fungi from the Great Smoky Mountains National Park, including three new species. Fungal Diversity: Accepted.

 

Jiao, Ping, Dale C. Swenson, James B. Gloer, Jinx Campbell, and Carol A. Shearer. 2006. Decaspirones A–E, bioactive spirodioxynaphthalenes from the Freshwater aquatic fungus Decaisnella thyridioides. Journal of Natural Products: http://pubs3.acs.org/acs/journals/doilookup?in_doi=10.1021/np060385k

 

Shearer, C.A., E. Descals, B. Kohlmeyer, J. Kohlmeyer, L. Marvanová, D. Padgett, D. Porter, H. Raja, J.P. Schmit, H. Thornton, & H. Voglmayr. 2007. Fungal biodiversity in aquatic habitats. Biodiversity and Conservation 16: 49-67.

 

Campbell, J., A. Ferrer, H. A. Raja, S. Sivichai, C. A. Shearer. 2007. Evolutionary relationships among taxa in the Jahnulales inferred from nuclear ribosomal DNA sequences. Canadian Journal of Botany 85: 873–882.

 

Ferrer, A. and C.A. Shearer. 2007. Three new species of Luttrellia from temperate and tropical freshwater habitats. Mycologia 99: 144–151

 

Ferrer, A. and C.A. Shearer. 2007. Megalohypha, a new genus in the Jahnulales from aquatic habitats in the tropics. Mycologia 99: 456–460.

 

Raja, H.A and C.A. Shearer. 2007. Freshwater ascomycetes: Aliquandostipite minuta (Jahnulales, Dothideomycetes), a new species from Florida. Mycoscience 48: 395–398.

 

Ferrer, A. and C.A. Shearer. 2008. Lucidascocarpa pulchrum, a new ascomycete genus and species from freshwater habitats in the American tropics. Mycologia: Accepted.

 

Raja H.A. and Miller A. N. and Shearer C.A. 2008. Freshwater ascomycetes: Aquapoterium pinicola, a new genus and species of Helotiales (Leotiomycetes) from Florida. Mycologia 100:141–148.

 

Ferrer, A. Raja H.A. and C.A. Shearer. 2008. Lucidascocarpa pulchra a new ascomycete genus and species from freshwater habitats in the tropics. Mycologia 100: 642–646.

 

Raja, H.A. and C.A. Shearer. 2008. Freshwater Ascomycetes: new species from aquatic habitats in Florida. Mycologia 100:467¬–489.

 

Simonis, J.L., H. Raja & C.A. Shearer. 2008. Extracellular enzymes and soft-rot decay: Are ascomycetes important degraders in freshwater? Fungal Diversity 31:135–146.

 

Raja H.A., Carter A., Platt H.W. and Shearer C.A. 2008. Freshwater ascomycetes: Jahnula apiospora (Jahnulales, Dothideomycetes), a new species from Prince Edward Island, Canada. Mycoscience 49:326–328.

 

Barbosa F. R., Gusmoa L.R.P. Raja H.A. and Shearer C.A. 2008. Annulatascus apiculatus sp. nov., a new freshwater ascomycete from semi-arid caatinga biome of Brazil. Mycotaxon 106: 403-407.

 

Raja H.A., Schmit J.P. and Shearer C.A. 2009. Latitudinal, habitat and substrate distribution patterns of freshwater ascomycetes in the Florida peninsula. Biodiversity and Conservation 18: 419–455.

 

Shearer C.A., Raja H.A., Miller A.N., Nelson P., Tanaka K., Hirayama K., Marvanová L., Hyde K.D., and Zhang Y. 2009. The molecular phylogeny of freshwater Dothideomycetes. Studies in Mycology 64: 145–153.

 

Schoch C.L., Crous P.W., Groenewald J.Z., Barrés B., Boehm E.W.A., De Gruyter J., de Hoog G.S., Dixon L.J., Fournier J., Grube M., gueidan C., Harada Y., Hatakeyama S., Hirayama K., Hosoya T., Hyde K.D., Jones E.B.G., Kohlmeyer J., Lücking R., Lumbsch H.T., Lutzoni F., Marvanová, Mbatchou J.S., Miller A.N., Mugambi G.K., Muggia L., Nelson M.P., Nelson P., Ownesby C.A.,Phongpaichit S., Pointing S.B., Pujade-Renaud V., Raja H.A., Plata E.R., Robbertse B., Ruibal C., Sakayaroj J., Sano T., Selbmann L., Shearer C.A., Shirouzu T., Slippers B., Suetrong S., Tanaka K., Volmkann-Kohlmeyer B., Wood A.R., Woudenberg J.H.C., Yonezawa H., Zhang Y., and Spatafora J.W. 2009. A class-wide phylogenetic assessment of Dothideomycetes. Studies in Mycology 64: 1-15.

 

Hosoe T., Gloer J.B., Wicklow D.T., Raja H.A., and Shearer C.A. 2010. New nonadride analogues from an undescribed fungus belonging to the order Pleosporales. Heterocycles 81: 2123–2130.

 

Hosoe T., Gloer J.B., Raja H.A., and Shearer C.A. 2010. Radicinol analogs from the freshwater aquatic fungus Xylomyces chlamydosporus. Mycotoxins 60: 1–6.

 

Hirayama K., Tanaka K., Raja H.A., Miller A.N. and Shearer C.A. 2010. A molecular phylogenetic assessment of Massarina ingoldiana. Mycologia 102 (3): 729–746.

 

Raja H.A., Ferrer A., Miller A.N. and Shearer C.A. 2010. Freshwater ascomycetes: Wicklowia aquatica, a new genus and species in the Pleosporales from Florida and Costa Rica. Mycoscience 51: 208–214.

 

Raja H.A., Miller A.N., and Shearer C.A. 2010. Freshwater Ascomycetes: Hyalorostratum brunneisporum, a new genus and species in the Diaporthales (Sordariomycetidae, Sordariomycetes) from North America. Mycosphere 1(4): 275–284.

 

Raja H.A., Violi H.A. and Shearer C.A. 2010. Freshwater ascomycetes: Alascospora evergladensis, a new genus and species from the Florida Everglades. Mycologia 102: 33–38.

 

Raja H.A., Hirayama K., Tanaka K., Miller A.N., and Shearer C.A. 2011. Freshwater Ascomycetes: Two new species of Lindgomyces (Lindgomycetaceae, Pleosporales, Dothideomycetes) from Japan and USA. Mycologia 103: 1421–1432.

 

Zelski S.E., Raja H.A., Miller A.N., Barbosa F.R., Gusmão L.F.P., and Shearer CA. 2011. Chaetorostrum quincemilemensis, gen. et sp. nov., a new freshwater ascomycete and its Taeniolella-like anamorph from Peru. Mycosphere 2: 593-600 Doi 10.5943/mycosphere/2/5/9.

 

Zelski S.E., Raja H.A., Miller A.N., and Shearer C.A. 2011. Longicollum biappendiculatum gen. et sp. nov., a new freshwater ascomycete from the Neotropics. Mycosphere 2: 539–545.

 

Ferrer, A., Miller, A. and Shearer, C.A. 2011. Minutisphaeria and Natipusilla: Two new genera of freshwater Dothideomycetes. Mycologia 103: 411-23.

 

Raja H.A., Schoch C.L. Hustad V.P., Shearer C.A., and Miller A.N. 2011. Testing the phylogenetic utility of MCM7 in the Ascomycota. Mycokeys 1: doi: 10.3897/mycokeys.1.1966.

 

Raja H.A., Jacques F., Shearer C.A., and Miller A.N. 2012. Freshwater Ascomycetes: Coniochaeta gigantospora sp. nov. based on morphological and molecular data. Mycoscience DOI 10.1007/s10267-012-0181-4.

 

Raja H.A., Miller A.N., and Shearer C.A. 2012. Freshwater Ascomycetes: Natipusillaceae, a new family of tropical fungi, including Natipusilla bellaspora sp. nov. from the Peruvian Andes. Mycologia 104: 569–573.

 

Schoch, C.L., K.A. Seifert, S. Huhndorf, V. Robert, J.L. Spouge, C.A. Levesque, W. Chen and Fungal Barcoding Consortium*. 2012. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Published online before print March 27, 2012, doi: 10.1073/pnas.1117018109 PNAS March 27, 2012 (Shearer member of Fungal Barcoding Consortium.

 

Caballero-George, Catherina, Thomas Sorkalla, Daniel Jakobs, Jessica Bolaños, Huzefa Raja, Carol Shearer, Eldredge Bermingham, and Hanns Häberlein. 2012. Fluorescence Correlation Spectroscopy in Drug Discovery: Study of Alexa532-Endothelin 1 Binding to the Endothelin ETA Receptor to Describe the Pharmacological Profile of Natural Products. 2012: Article ID 524169, 7 pages.

 

Barbosa F.R., Gusmao L.F., Raja H.A. and Shearer C.A. 2012. New species and new records of freshwater ascomycetes from Brazil and Costa Rica. Mycologia. 2012 Oct 18. [Epub ahead of print]

 

Barbosa F.R., Raja H.A., Gusmao L.F., and Shearer C.A. 2012. Three Chaetomium species (Chaetomiaceae, (Ascomycota) from the semi-arid region of Brazil. Sitientibus série Ciências Biológicas 12:115–118.

 

Ferrer A, Miller AN, Sarmiento C and Shearer CA. 2012. Three new genera representing novel lineages of Sordariomycetidae (Sordariomycetes, Ascomycota) from tropical freshwater habitats in Costa Rica. Mycologia 104: 865-879.

 

Raja H.A., Tanaka K., Hirayama K., Hashimoto A., Oberlies N.H., Figueroa M., Miller A.N., Zelski S.E., Shearer C.A. 2013. Freshwater Ascomycetes: Minutisphaera (Dothideomycetes) revisited, including one new species from Japan. (Mycologia).

 

Raja H.A., Oberlies N.H., Tamam-El Elimat, Miller A.N., Zelski S.E., Shearer C.A. 2013. Lindgomyces angustiascus, (Lindgomycetaceae, Pleosporales, Dothideomycetes), a new species from freshwater habitats in the USA, Mycoscience: In Press. http://dx.doi.org/10.1016/j.myc.2012.12.004


 

Shearer, C.A., P. Fallah, A. Ferrer and H. Raja. 2008. Freshwater Ascomycete Database: http://fm5web.life.uiuc.edu:23523/ascomycete/  (Continuously updated).