Well, our gel has now finished running. A gel this size probably ran about 30 minutes at roughly 100 V in TBE buffer to advance to this point. Remember what we learned about the two different dyes? Can you see the difference in migration distance?
The blue arrow shows the location of the "leading dye," the one which advanced the farthest. Always orient yourself by looking for the wells at one end of the gel. The red arrow marks the location of the "trailing dye," the dye that ran more slowly. Our DNA lies hopefully somewhere in between those markers. How do we find out for sure? Go on to the next photo!!
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