Lecture Titles, Activities, and Exam Dates - Spring 2009
| Lecture | Date | Day | Lecture topic(s) and activities | Files |
|---|---|---|---|---|
| 1. | 1/20 | Tu | Course Introduction; Structure and properties of monosaccharides and their derivatives |
Lecture_1A.pdf Lecture_1B.pdf |
| 2. | 1/22 | Th | Glycosidic linkages: formation, anomeric configuration | Lecture_2.pdf |
| 3. | 1/27 | Tu | Polysaccharides and proteoglycans: structure, properties, occurrence | Lecture_3.pdf |
| 4. | 1/29 | Th | Glycoconjugates: structure, properties, and occurrence of glycoproteins and glycolipids. QUIZ 1 |
Lecture_4.pdf |
| 5. | 2/3 | Tu | Donors and carriers in glycan biosynthesis: sugar nucleotides and
polyisoprenoid-linked sugars and their metabolic origin HOMEWORK 1 ASSIGNED. |
Lecture_5.pdf |
| 6. | 2/5 | Th | Glycosyltransferase and hydrolase enzymology; inverting/retaining mechanisms; glycosyltransferase features and proteomics. | Lecture_6.pdf |
| 7. | 2/10 | Tu | Glycan assembly in Bacteria and Archaea I:
peptidoglycan, lipopolysaccharides, exopolysaccharides HOMEWORK 1 DUE. |
Lecture_7.pdf |
| 8. | 2/12 | Th | Glycan assembly in Bacteria and Archaea II: transmembrane translocation steps; protein glycosylation; mycobacterial glycan assembly. QUIZ 2. |
Lecture_8.pdf |
| 9. | 2/17 | Tu | Glycan assembly along the eukarytic secretory pathway I: dolichol pathway for N-glycosylation in the endoplasmic reticulum (ER); evolutionary parallels with Bacteria and Archaea HOMEWORK 2 ASSIGNED. |
Lecture_9.pdf |
| 10. | 2/19 | Th | Glycan assembly along the eukaryotic secretory pathway II: glycolipid anchoring O-glycosylation, C-mannosylation in the ER |
Lecture_10.pdf |
| 11. | 2/24 | Tu | Glycan assembly along the eukarytic secretory pathway III: O-glycosylation and N-linked glycan processing in the Golgi; polysialic acid; yeast mannan; sugar nucleotide transport. HOMEWORK 2 DUE. |
493_Lectures_11+12.pdf |
| 12. | 2/26 | Th | Nuclear and cytoplasmic glycosylation: O-linked N-acetylglucosamine,
a regulatory alternative to phosphorylation QUIZ 3. |
|
| 13. | 3/3 | Tu | Polysaccharide and glycosaminoglycan synthesis: processivity and
direction of chain growth HOMEWORK 3 ASSIGNED. |
493_Lecture_13.pdf |
| 14. | 3/5 | Th | Biosynthesis of cellulose and chitin; biosynthetic issues in formation of
β1,4-linked polymers. |
493_Lecture_14.pdf |
| 15. | 3/10 | Tu | Glycosylhydrolases and glycan degradation |
493_Lecture_15.pdf |
| 16. | 3/12 | Th | Protein-carbohydrate interactions; lectins, selectins, siglecs QUIZ 4. |
493_Lecture_16.pdf |
| 17. | 3/17 | Tu | N-linked glycans in protein folding and stability ("quality control") HOMEWORK 3 DUE. |
493_Lecture_17.pdf |
| 3/19 | Th | EXAM 1 |
Exam 1 key.pdf |
|
| 3/24 and 3/26 | SPRING BREAK | |||
| 18. | 3/31 | Tu | Glycans transport: lysosomal targeting & storage diseases; glycolipid anchored proteins and membrane "rafts" lymphocyte homing |
493_Lecture_18.pdf |
| 19. | 4/2 | Th |
Glycans in animal development and immunity; altered glycosylation and tumor progression;
glycosylation defects in rheumatoid arthritis and autoimmune disease QUIZ 5. |
493_Lecture_19.pdf |
| 20. | 4/7 | Tu | Congenital Disorders of Glycosylation HOMEWORK 4 ASSIGNED. |
493_Lecture_20.pdf |
| 21. | 4/9 | Th | Glycans in infectious disease: microbial adhesion, bacterial toxins; glycosyltransferases as antimicrobial drug targets | 493_Lecture_21.pdf |
| 22. | 4/14 | Tu | Glycans in plant-legume symbioses: rhizobial signalling oligosaccharides and possible vertebrate counterparts | 493_Lecture_22.pdf |
| 23. | 4/16 | Th |
Chemical approaches to synthesize and modify glycans; carbohydrate arrays QUIZ 6. |
493_Lecture_23.pdf |
| 24. | 4/21 | Tu | Glycosylation and protein expression; "humanizing" yeast glycoprotein structure HOMEWORK 4 DUE. |
493_Lecture_24.pdf |
| 25. | 4/23 | Th | GRAD STUDENT PRESENTATION | 493_Lecture_25.pdf |
| 26. | 4/28 | Tu | GRAD STUDENT PRESENTATION | 493_Lecture_26.pdf |
| 27. | 4/30 | Th | GRAD STUDENT PRESENTATION | 493_Lecture_27.pdf |
| 28. | 5/5 | Tu | Polysaccharides as biofuels | 493_Lecture_28.pdf |
| 5/13 | W | FINAL EXAM 8:00–11:00 a.m. | 493_Exam_2_Ans.pdf | |