MCB 428
Microbiology
School of Molecular and Cellular Biology
University of Illinois at Urbana-Champaign
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Fall 2018 Syllabus

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Course Instructor:
Prof. Jack Ikeda

Office location TBA

(217) 244-8732
jikeda@illinois.edu

                      

Teaching Assistant:

Alexander Asilador



To request disability accommodations, please speak to the instructor after class or make an appointment by email.

*Asterisk by date indicates that a Pre-Lab Quiz is due that day.

Date Topic Pages

 

Aug 28

 

Course Introduction: Orientation, policies, safety practices

 

i-xxv, 1-23

Aug 30

Sept 4

 

Introduction to Bacterial Pathogens: Basic lab skills

Basic lab skills

6-23

 

Exercise 1: Urinary Tract Infections 24-40
*Sept 6 Day 1: Dipstick test; Gram stain; streak plate
Sept 11 Day 2: Gram stain: oxidase test; biochemical tubes; Sensititre
Sept 13 Day 3: Read results; streak for MBC test
Sept 18 Day 4: Read MBC plates
 
Lab Report due Sept 25; Post-Lab 1 Urinary: Sept 26
 
Exercise 2: Infections of Gram-Positive Cocci 41-66
*Sept 20 Day 1: Throat swab; Group A Strep test; streak BAP; check blood bottles
Sept 25 Day 2: Gram stain; catalase and coagulase test; phage typing
Sept 27 Day 3: Streptocard Strep test, E-tests; read phage typing results
   
 
Lab Report due Oct 4; Post-Lab 2 Gram-Positive: Oct 5
 
Exercise 3: Respiratory Tract Infections 67-87
*Oct 2 Day 1: Gram stain; streak plates
Oct 4 Day 2: Normal flora; read results; Gram stain; Enterotube; quad plate
Oct 9 Day 3: Read results; read HAI plates
 
Lab Report due Oct 16; Post-Lab 3 Respiratory: Oct 17
     
Oct 11 EXAM 1: Introduction and Exercises 1 and 2
Section A 10:00 am to 11:50 am in 218 Noyes Lab
Section B 1:00 pm to 2:50 pm in 218 Noyes Lab
 
 
Exercise 4: Gastrointestinal Infections 88-113
*Oct 16 Day 1: Gram Stain; Streak plates
Oct 18 Day 2: Score plates; streak plates; Gram stain; inoculate & perform tests
Oct 23 Day 3: Gram stain; score plates & tests; inoculate biochemical tests
Oct 25 Day 4: Score tests
 
Lab Report due Oct 30; Post-Lab 4 GI: Oct 31
 

Exercise 5: Transfer of Antibiotic Resistance Genes

114-131
*Oct 30 Day 1: Mating of donor and recipient; quantitating plasmid transfer
Nov 1 Day 2: Check conjugation; comparison of plasmids
  Lab Report due Nov 8; Post-Lab 5 Transfer: Nov 9
 
Nov 6 EXAM 2: Exercises 3 and 4
 

Section A 10:00 am to 11:50 am in 218 Noyes Lab
Section B 1:00 pm to 2:50 pm in 218 Noyes Lab

 
     
  Exercise 6: Anaerobic Bacterial Infections 132-155
*Nov 8 Day 1: Gram stain; streak plates
Nov 13 Day 2: Read plate results; Gram stain; quad plate
Nov 15 Day 3: Read results
Lab Report due Nov 27; Post-Lab 6 Anaerobes: Nov 28
     
Nov 19-23 Thanksgiving Holiday
     
  Exercise 7: Sexually Transmitted Infections 156-190
*Nov 27 Day 1: Read Gonorrhea, Chlamydia, RPR, HPV and HSV tests
Nov 29 Day 2: Read HIV ELISA, Western blot and HBV tests
  Practice and review for Lab Practical
  Lab Report due Dec 4; Post-Lab 7 STI: Dec 7
 
  Lab Practical Exam  
Dec 4 Day 1: Gram stain; Streaking; Microscopy
Dec 6 Day 2: Biochemical tests
   
Dec 11 Lab Cleanup
 
Finals Week EXAM 3 (Final Exam) TBA: Exercises 5, 6 and 7