MCB 421
Microbial Genetics

School of Molecular and Cellular Biology
University of Illinois at Urbana-Champaign

MCB 421 Lecture Handouts

Tue Aug 23	The Salvation of Doug
Thur Aug 25	A cartoon overview of transcription and translation Rho-independent transcription termination Rho dependant polarity
Tue Aug 30	Mismatch repair Types of Mutations Frameshift Mutations Deletions and insertion mutations Mutation rates Hydroxylamine 5 BU
Thur Sept 1	Example of Some Useful. Mutagens The Zen of Mutagenesis Strategies for finding mutants Screens Enrichments How Nonsense Mutations got their names
Tue Sept 6	Kuzminov Lecture DOC PDF Lambda Excision Complex DOC PDF
Thur Sept 8	Mutator Strains PDF Isolate Mutations Continued[PPTX] Isolation of Mutants[PPTX]
Tue Sept 13	Synthetic Lethals Suppressor Analysis Intragenic Suppressors
Thur Sept 15	Intergenic Interaction Suppressors An Example of Bypass Suppression Frameshift suppressor Suppressor tRNAs Nonsense suppressors Nonsense mutations
Tue Sept 20	Example of cis dominance
Thur Sept 22	Phage Lytic growth: Phage P22 packaging Lambda vs P22 replication and packaging Generalized transduction: Packaging of generalized transducing phage Abortive transduction
Tue Sept 27	No handouts
Thur Sept 29	No handouts
Tue Oct 4	Phage: What are phage (link to ASM site) Why study phage? Some important biological lessons learned from studying phage Phage Cartoon Lytic growth: Formation of plaques Lysogeny: Lysogenic conversion - Lysogenic phage and bacterial virulence
Thur Oct 6	Genetic Exchange Host Restriction Systems Genetics of host restriction systems Antirestriction systems Restriction Modification
Tue Oct 11	Phage Lysogeny: Lambda Gene organization The lysis-lysogeny decision Homoimmune vs heteroimmune lysogens Lambda att sites OR Figure CTX Phage
Thur Oct 13	Phage Specialized transduction: Specialized transducing particles Formation of lambda specialized transducing phage Formation of lambda LFT and HFT lysates Transduction with a lambda dgal HFT lysate An example Additional File: Model for Excisive Intasome: Figure PDF
Tue Oct 18	No handouts
Thur Oct 20	Transposable elements Insertion sequences and transposons: Types of transposable elements Replicative transposition Non-replicative transposition Animation of Tn7 transposition (via Nancy Craig's lab) Regulation of transposition Phage Mu Conjugal transposons Replictative Transposons
Tue Oct 25	Transposable elements Transposon derivatives: Some useful Mu derivatives (Mud) Portable regions of homology: Polarity of transposon insertions Genetic regulation Reporter genes Operon and gene fusions Differences between operon and gene fusions Use of fusions to distinguish Transcriptional vs translational regulation
Thur Oct 27	No handouts
Tue Nov 1	Courtesy of Dr. Abigail Salyers: Lecture PPT, Lecture PDF , RNA PDF
Thur Nov 3	In vitro genetic tricks Site-directed mutagenesis; Using site-directed mutagenesis to test sequence motifs; Codon usage; Codon usage in E. coli: Rare codons IntDOT primer design PPT pUC19 Map PDF pet29 map and restriction sites PDF pet28 map and restriction sites PDF Polymerase chain reaction (PCR)
Tue Nov 8	Red Mediated Recombination Amino Acids Allele Replacement Allele Replacement 2
Thur Nov 10	Developing a new genetic system in bacteria
Tue Nov 15	No handouts
Thur Nov 17	No handouts
Nov 21 - 27 Thanksgiving Break	No handouts
Tue Nov 29	Conjugative Transposons, Lecture PDF
Thur Dec 1	No handouts
Tue Dec 6	No handouts
Wed, Dec 14	No handouts

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