MCB 301
Experimental Microbiology
School of Molecular and Cellular Biology
University of Illinois at Urbana-Champaign
course home pagecourse contactUniversity of Illinois

 
MCB 301 Syllabus: Fall 2018 (Downloadable PDF)

Course Web Site: http://www.life.uiuc.edu/mcb/301

Course Instructor:
Dr. Jack Ikeda
164A Burrill Hall
244-8732
jikeda@illinois.edu
Laboratory Coordinator:
Renee L. Alt
232A Burrill Hall
333-4306 (Office)
rlalt@illinois.edu
Dr. Ikeda
Office Hours

MW: 1:00 2:00 pm
(or by appointment)
Ms. Alt
Office Hours

By appointment only

Course Description: Laboratory emphasizing the fundamentals of microbiology. Topics include growth, isolation, and identification of bacteria; antibiotic resistance; ELISA; conjugation and transposon mutagenesis.

Credit:3 credit hours. Students must register for one lab-discussion and one lecture section.

Prerequisite: Students are expected to have taken MCB 250 and MCB 251 or course equivalents prior to enrolling in MCB 301. Students should also have credit or concurrent registraton in MCB 300, or consent of instructor.

Required Course Materials:

  1. “Experimental Microbiology: Laboratory Exercises for MCB 301, Fall 2018 ”.  Gail K. Grabner, Jack Ikeda and Renee L. Alt.
  2. “Biology Laboratory Notebook” Carbonless with duplicate, numbered pages
  3. Ruler and non-graphing Scientific Calculator, required at every lab and exam

Course Policies:
All students are assumed to have read and understood the “Student Code 2018-2019” University of Illinois, and will be expected to act accordingly.
The Code is available online at: http://www.admin.uiuc.edu/policy/code/index.html

Contacting MCB Course Personnel:

  • MCB course personnel are more than happy to assist students.
  • Emails to instructors, TAs, or course coordinators will only be answered if they come from an @illinois.edu account. We will only use this account in order to protect your educational information and profile. As a student, please remember that when you email a staff member, it is important to include all pertinent information so that we can assist you in the most efficient and effective manner possible. This information includes:
    • The course rubric in the subject line
    • Your full first and last name
    • Your NetID (the first part of your illinois.edu email account)
    • Your UIN (9 digit number that can be found on your ICard)
    • The course that you are concerned about (the course personnel often work with multiple courses)
    • Your section letter/number
    • The previous email "thread" or previous communicated information pertinent to the situation
  • Your cooperation will help us respond much more quickly to your concerns.
Social Media Use
  1. Use of any social or electronic media to share course information, request course information or make confidential course information public is prohibited. Any use of this type may earn you a zero or a more extreme penalty at the discretion of the instructor on an assignment or exam.

  2. Any social media sites created in relation to MCB courses must grant access to course personnel upon request. Failure to provide access will result in a failing grade in the course for the group/site's administrator(s).
Course Recordings
  1. Students are welcome and encouraged to make audio recordings of course lectures.

  2. The material recorded is intellectual and copyrighted property of the University of Illinois Board of Trustees and may be made for personal use only.

  3. Video recordings of any kind are strictly prohibited.

  4. Posting of audio recordings or transcriptions on social or electronic media platforms is strictly prohibited.

Religious Observances and Practices:

  • Students are required to submit the Request for Accommodation for Religious Observances Form (which can be found at www.odos.illinois.edu/.../Religious_Observance_Accommodation_Request_ Form.docx) to their instructors and the Office of the Dean of Students requesting accommodation by the end of the second week of the course. Requests that are not submitted within this time frame may not be granted.

DRES Accommodations:

  • If a student has DRES (Disability Resources and Educational Services) accommodations, documentation must be submitted to course personnel by the end of the second week of class.
  • If a student believes that they need DRES accommodations, they should contact DRES at disability@illinois.edu.

Course Syllabus
Laboratory Schedule   Lecture Schedule   Assignment Schedule


Laboratory Schedule

MCB 301 Syllabus Fall 2018

Date (pt total) Experiment Lab Manual Pages
Day 1
Mon Aug 27
Tu Aug 28
Course introduction
1: Streak Plate
I-XXXIX
1-9

Day 2
Wed Aug 29
Thu Aug 30
1, 2: Evaluations
3: Spread plate
4: Simple stain
8
10-13
14-25
Mon Sept 3
Tu Sept 4
Labor Day - NO CLASS
Day 3
Wed Sept 5
Thu Sept 6
3: Evaluations
5: Gram stain
13
25-26
Day 4
Mon Sept 10
Tue Sept 11
6: Wet mount, Darkfield and Phase Contrast

29-38
Day 5 (13)
Wed Sept 12
Thu Sept 13
6a: Sporeformers enrichment

39-50

Day 6 (13)
Mon Sept 17
Tue Sept 18
6b: Sporeformers isolation, 1
51-52
Day 7 (12)
Wed Sept 19
Thu Sept 20

6b: Sporeformers isolation, 2

53-54
Day 8 (13)
Mon Sept 24
Tue Sept 25
6c: Sporeformers post-enrichment, 1
55-56
Day 9 (12)
Wed Sept 26
Thu Sept 27
6c: Sporeformers post-enrichment, 2
56-58
Day 10 (26)
Mon Oct 1
Tue Oct 2
8a: Identification isolation
10: Antibiotic Producing Bacteria, Intro

71-79

Day 11 (46)
Wed Oct 3
Thu Oct 4
8b: Identification purification, 1
9a-c: Biochemical tests, 1
10: Antibiotic Producing Bacteria
81
87-116
Thurs Oct 4 OPEN HOURS BEGIN
SEE SCHEDULE
 
Day 12 (47)
Mon Oct 8
Tue Oct 9
8b: Identification purification, 1
9a-c: Biochemical tests, 2
10: Antibiotic Producing Bacteria
81
87-116
Day 13 (25)
Wed Oct 10
Thu Oct 11
8c: Identification dichotomous keys; inoculate tests, 1
10: Antibiotic Producing Bacteria
82-83

 

Day 14 (25)
Mon Oct 15
Tue Oct 16
8c: Identification; inoculate tests, 2
10: Antibiotic Producing Bacteria

82-83

Day 15 (25)
Wed Oct 17
Thu Oct 18
8c: Identification; inoculate tests, 3
10: Antibiotic Producing Bacteria

82-83

Fri Oct 19 OPEN HOURS END  
Day 16 (17)
Mon Oct 22
Tue Oct 23
10: Antibiotic Producing Bacteria

 

Day 17 (17)
Wed Oct 24
Thu Oct 25
10: Antibiotic Producing Bacteria

 

Day 18 (17)
Mon Oct 29
Tue Oct 30
10: Antibiotic Producing Bacteria

Day 19 (17)
Wed Oct 31
Thu Nov 1
10: Antibiotic Producing Bacteria

Day 20 (17)
Mon Nov 5
Tue Nov 6
10: Antibiotic Producing Bacteria
Day 21 (29)
Wed Nov 7
Thu Nov 8
16a: Transposon mutagenesis conjugation
10: Antibiotic Producing Bacteria
118-122
Day 22 (29)
Mon Nov 12
Tue Nov 13
16c: Transposon mutagenesis selection, 1
10: Antibiotic Producing Bacteria
123
Day 23 (30)
Wed Nov 14
Thu Nov 15
16c: Transposon mutagenesis selection, 2
10: Antibiotic Producing Bacteria
124
Sat Nov 17 to Sun Nov 25
Fall Break  
Day 24 (30)
Mon Nov 26
Tue Nov 27
16c: Transposon mutagenesis characterization, 1
10: Antibiotic Producing Bacteria
125-129
Day 25 (30)
Wed Nov 28
Thu Nov 29
16c: Transposon mutagenesis characterization, 2
10: Antibiotic Producing Bacteria

125-129

Day 26
Mon Dec 3
Tue Dec 4
Lab Practical Review and Practice
Lab clean up & drawer inventory
TA ICES forms
 
Day 27 (42)
Wed Dec 5
Thu Dec 6
Lab Practical Exam, Part I
Dilution problem calculation, Gram stain, Brightfield microscopy, streak plate
Day 28 (58)
Mon Dec 10
Tue Dec 11
Lab Practical Exam, Part II
Dilutions & spread plate, wet mount & phase contrast, biochemical tests, dichotomous key

Lecture Schedule

Date Topic(s)
August 31
Lecture 1

Basic microbiology techniques (aseptic technique, pure culture, plating); Luminescent bacteria; Staining (cell wall structure)

September 7
Lecture 2

Staining; Microscopy

September 14
Lecture 3
Microscopy; Sporeformers (enrichments)
September 21
Exam 1
Basic microbiology techniques; Staining; Microscopy
September 28
Lecture 4
Sporeformers; Bacterial Growth
October 5
Lecture 5
Bacterial Growth;Identification; Biochemical Tests
October 12
Lecture 6
Identification; Biochemical Tests
October 19

Exam 2
Sporeformers; Bacterial growth

DROP DEADLINE

October 26
Lecture 7

Antibiotic producing bacteria

November 2
Lecture 8
Antibiotic producing bacteria
November 9
Lecture 9
Antibiotic producing bacteria Transposon mutagenesis
November 16
Exam 3
Identification; Biochemical tests
November 23
Fall Break
November 30
Lecture 10
Transposon mutagenesis; Dilution problem
December 7
Lecture 11
TBA

Finals Week

Final Exam
Antibiotic producing bacteria; Transposon mutagenesis

Assignment Schedule

Date Assignment Due
Point Total
Day 1
Mon Aug 27
Tu Aug 28
Nothing Due
Day 2
Wed Aug 29
Thu Aug 30
Nothing Due
Mon Sept 3
Tu Sept 4
Labor Day - NO CLASS
Day 3
Wed Sept 5
Th Sept 6
Pre-lab 1-5: Basic Technique
10 points
Day 4
Mon Sept 10
Tu Sept 11

Nothing Due

 

Day 5
Wed Sept 12
Thu Sept 13

Post-lab 1-5: Basic Technique
Pre-lab 6: Sporeforming bacteria

10 points
10 points

Day 6
Mon Sept 17
Tues Sept 18
Nothing Due

 

Day 7
WedSept 19
Thu Sept 20

Nothing Due

 

Fri Sept 21
Exam 1
Basic microbiology techniques; Microscopy; Staining;
50 points
Day 8
Mon Sept 24
Tue Sept 25

Nothing Due

Day 9
Wed Sept 26
Thu Sept 27

Nothing Due

 

Fri Sept 28

7: Growth Curve opens in LON-CAPA at 2pm

Day 10
Mon Oct 1
Tue Oct 2
Post-lab 6: Sporeforming bacteria
Pre-lab 8: Identification
10 points
10 points
Day 11
Wed Oct 3
Thu Oct 4
6: Sporeforming bacteria Lab Report
Pre-lab 9: Biochemical Tests
63 points
10 points
Day 12
Mon Oct 8
Tue Oct 9
7: Growth Curve exercise on LON-CAPA
10: Antibiotic Producing Bacteria, Protocol 1

40 points

Day 13
Wed Oct 10
Thu Oct 11
Pre-lab 10: Antibiotic Producing Bacteria Post-lab 9: Biochemical tests
10 points
10 points
Day 14
Mon Oct 15
Tue Oct 16
9: Biochemical Tests Lab Report

43 points

10 points

Day 15
Wed Oct 17
Thu Oct 18

10: Antibiotic Producing Bacteria, Protocol 2


Fri Oct 19
Exam 2
Sporeformers; Bacterial growth
50 points
Day 16
Mon Oct 22
Tu Oct 23
Post-lab 14: Identification
10: Antibiotic Producing Bacteria, Protocol 1 Final

10 points


Day 17
Wed Oct 24
Thu Oct 25

14: Identification Lab Report

47 points

Day 18
Mon Oct 29
Tue Oct 30

Nothing Due



Day 19
Wed Oct 31
Thu Nov 1

Nothing Due

 

Day 20
Mon Nov 5
Tue Nov 6
10: Antibiotic Producing Bacteria, Protocol 3
Day 21
Wed Nov 7
Thu Nov 8
Pre-lab 11: Transposon mutagenesis
10 points
Day 22
Mon Nov 12
Tue Nov 13

Nothing Due

 

Day 23
Wed Nov 14
Thu Nov 15
Nothing Due

Fri Nov 16

Exam 3
Ideintification; Biochemical tests
50 points
Sat Nov 17 to Sun Nov 25
Fall Break  
Day 24
Mon Nov 26
Tue Nov 27

Nothing Due

 

Day 25
Wed Nov 28
Thu Nov 29
Post-lab 10: Antibiotic producing bacteria

10 points

Day 26
Mon Dec 3
Tue Dec 4
10: Antibiotic Producing Bacteria, Final Paper (NO LATE LAB REPORTS ACCEPTED)

11: Transposon mutagenesis Lab Report (NO LATE LAB REPORTS ACCEPTED)

Post-lab 11: Transposon mutagenesis

62 points

 

10 points

Day 27
Wed Dec 5
Thu Dec 6
Lab Practical Exam, Part I
Dilution problem calculation, Gram stain, Brightfield microscopy, streak plate
Parts 1 and 2 worth a total of 100 points
Day 28
Mon Dec 10
Tue Dec 11
Lab Practical Exam, Part II
Dilutions & spread plate, wet mount & phase contrast, biochemical tests, dichotomous key

Finals Week

Final Exam
Antibiotic producing bacteria; Transposon mutagenesis
50 points

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