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Course Web Site: http://www.life.uiuc.edu/mcb/301
Course Instructor:
Dr. Jack Ikeda
383 Burrill Hall
244-8732
jikeda@life.illinois.edu |
Laboratory Coordinator:
Renee L. Alt
241 Burrill Hall
333-4306 (Office), 333-8057 (Lab)
rlalt@illinois.edu |
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Office Hours (Dr. Ikeda)
MW: 1:00 PM - 2:00 PM
(or by appointment) |
Office Hours (Ms. Alt)
Tu-Th: 10:30 AM - 11:30 AM
(or by appointment) |
Course Description: Laboratory emphasizing the fundamentals of microbiology. Topics include growth, isolation, and identification of bacteria; antibiotic resistance; Southern blot; ELISA; conjugation and transposon mutagenesis.
Credit:3 credit hours. Students must register for one lab-discussion and one lecture section.
Prerequisite: Laboratory emphasizing the fundamentals of microbiology. Topics include growth, isolation, and identification of bacteria; restriction endonuclease analysis of DNA, genetic cloning, and gene transfer. Computer methods are used for the identification of microorganisms and for the analysis of recombinant DNA molecules. Prerequisite: MCB 250 and 251 and credit or concurrent registration in MCB 300, or consent of instructor.
Required Course Materials:
- “Experimental Microbiology: Laboratory Exercises for MCB 301, Fall 2009 ”. Gail K. Grabner, Tina M. Knox and Jack Ikeda.
- “Biology Laboratory Notebook” Carbonless with duplicate, numbered pages
- Ruler and Calculator, required at every lab and exam
- i-clicker
Course Policies:
All students are assumed to have read and understood the “Code of Policies and Regulations Applying to All Students,” University of Illinois, and will be expected to act accordingly.
The Code is available online at: http://www.admin.uiuc.edu/policy/code/index.html
Attendance Policies:
Consult with Lab Coordinator, Renee Alt, about any course absences or conflicts.
All students are assumed to have read and understood attendance policies printed in the lab manual.
Disabilities and Religious Observances:
Please contact the course coordinator during the first week of classes to make requests for disability accommodations or observation of religious holidays.
Fall 2009 Syllabus: Word pdf
Course Syllabus
Laboratory Schedule
An experiment highlighted in bold type indicates a pre-lab is due for that exercise prior to the lab period
| Date |
Experiment |
Lab Manual Pages |
Day 1
Mon Aug 24
Tu Aug 25 |
Course introduction
1: Streak Plate
2: Luminescent bacteria |
i-xvi
1-9
10-14
|
Day 2
Wed Aug 26
Th Aug 27 |
Course introduction
1, 2: Evaluations
3: Spread plate
4: Simple stain |
i-xvi
8, 13
15-19
|
Day 3
Mon Aug 31
Tu Sept 1 |
3: Evaluations
5: Gram stain |
18
33-35 |
Day 4
Wed Sept 2
Th Sept 3 |
6: Wet mount, Darkfield
7a-d: Enzymes and adaptation, 1 |
36-46
47-58 |
Mon Sept 7
Tu Sept 8 |
Holiday |
|
Day 5
Wed Sept 9
Th Sept 10 |
7a-d: Enzymes and adaptation, 2
8a: Lactic acid becteria enrichment9a-c: Biochemical tests, 1 |
47-58
59-67
73-113 |
Day 6
Mon Sept 14
Tu Sept 15 |
8b: Lactic acid bacteria isolation
9a-c: Biochemical tests, 2 |
68-69
73-114 |
Day 7
Wed Sept 16
Th Sept 17 |
8c: Lactic acid bacteria observation
10: Growth Curve, lecture |
70-72
115-127 |
Day 8
Mon Sept 21
Tu Sept 22 |
11: Snyder test, 1
12: Environmental effects (a-b), 1 |
128
131-144 |
Day 9
Wed Sept 23
Th Sept 24 |
11: Snyder test, 2
12: Conditional effects (a, b), 2
12: Conditional effects (c-e), 1 |
129-130
138-144
145-148 |
Day 10
Mon Sept 28
Tu Sept 29 |
12: Conditional effects (c-e), 2
13a: Sporeformers enrichment |
146, 148-156
157-168 |
Day 11
Wed Sept 30
Th Oct 1 |
13b: Sporeformers isolation, 1 |
169-170 |
Day 12
Mon Oct 5
Tu Oct 6 |
13b: Sporeformers isolation, 2
14a: Identification isolation |
171-173
181-189 |
Day 13
Wed Oct 7
Th Oct 8 |
13c: Sporeformers post-enrichment, 1
14b: Identification purification, 1
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173-174
190-191
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Th Oct 8 |
OPEN HOURS BEGIN, SEE SCHEDULE |
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Day 14
Mon Oct 12
Tu Oct 13 |
13c: Sporeformers post-enrichment, 2
14b: Identification purification, 2 |
174-180
191 |
Day 15
Wed Oct 14
Th Oct 15 |
14b: Identification purification, 2
15c: Identification dichotomous keys; inoculate tests, 1
18a: Southern blot electrophoresis
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191
192-196
197-212 |
Day 16
Mon Oct 19
Tu Oct 20 |
14c: Identification inoculate/score tests, 2
15b: Southern blot transfer
18b: Southern blot transfer |
192-196
213-219 |
Day 17
Wed Oct 21
Th Oct 22 |
14c: Identification inoculate/score tests, 3
15c: Southern blot hybridization
16a: ELISA fluid exchange
16b: ELISA analysis, 1 |
192-196
220-225
234-240
240-242 |
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Fri Oct 23 |
OPEN HOURS END |
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Day 18
Mon Oct 26
Tu Oct 27 |
15d: Southern blot washing
17b: ELISA analysis, 2
Agglutination (demo) |
226-227
242-243
244-250 |
Day 19
Wed Oct 28
Th Oct 29 |
15e: Southern blot detection |
228-231
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Day 20
Mon Nov 2
Tu Nov 3 |
15f: Southern blot analysis
17a-b: MIC determination, 1 |
232-233
251-256 |
Day 21
Wed Nov 4
Th Nov 5 |
21a-b: MIC determination, 2
18a: Transposon mutagenesis conjugation |
255-258
259-263 |
Day 22
Mon Nov 9
Tu Nov 10 |
18a: Transposon mutagenesis selection, 1
19: pGLO transformation, 1 |
264
273-275
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Day 23
Wed Nov 11
Th Nov 12 |
18b: Transposon mutagenesis selection, 2
19: pGLO transformation, 2 |
265
275-277 |
Day 24
Mon Nov 16
Tu Nov 17 |
18c: Transposon mutagenesis characterization, 1
20: Analysis of sore throat, 1 |
266-268
278-279
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Day 25
Wed Nov 18
Th Nov 19 |
18c: Transposon mutagenesis characterization, 2
20: Analysis of sore throat, 2 |
269-272
279-281 |
Mon Nov 23 to
Fri Nov 27 |
THANKSGIVING BREAK - NO CLASSES |
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Day 26
Mon Nov 30
Tu Dec 1 |
20: Analysis of sore throat, 3
Lab Practical Review
Lab clean up & drawer inventory |
282-284
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Day 27
Wed Dec 2
Th Dec 3 |
Lab Practical Exam, Part I
Dilution problem calculation, Gram stain, Brightfield microscopy, streak plate |
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Day 28
Mon Dec 7
Tu Dec 8 |
Lab Practical Exam, Part II
Dilutions & spread plate, wet mount & phase contrast, biochemical tests, dichotomous key |
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Lecture Schedule
| Date |
Topic(s) |
| August 28 |
Pure culture, aseptic technique, plating techniques, cell wall structure, staining, microscopy |
| September 4 |
Enzymes and Adaptation; Lactic acid bacteria |
| September 11 |
Biochemical Tests |
| September 18 |
Exam 1
Aseptic technique, Pure culture, Plating, Luminescent bacteria, Staining, Mictoscopy, Enzymes and Adaptation, Lactic acid bacteria, Biochemical tests |
| September 25 |
Snyder test, Conditional Effects |
| October 2 |
Sporeformer bacteria; Sporeformer metabolism and post-enrichment |
| October 9 |
Identification and dichotomous keys; Southern digest and electrophoresis |
| October 16 |
Exam 2
Snyder test, Growth curve, Conditional effects; Sporeformers |
| October 23 |
Southen transfer, probe synthesis and hybridization; ELISA and agglutionation |
| October 30 |
Southern washing, detection and analysis; MIC determination; Conjugation and pRL27 plasmid |
| November 6 |
Transposon mutagenesis and cell types; Transposon mutagenesis selection, counter selection; pGLO |
| November 13 |
Transposon mutagenesis characterization, Analysis of sore throat |
| November 20 |
Lab practical review; ICES forms for Dr. Ikeda |
| November 27 |
Thanksgiving Break |
| December 4 |
Exam review |
| Finals Week |
Exam 3
Identification, pGLO, ELISA and agglutination, Southern blot (a-f), Sore throat, MIC, Transposon mutagenesis |
Assignment Schedule
| Date |
Assignment Due |
Point Total |
Day 1
Mon Aug 24
Tu Aug 25 |
Nothing Due |
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Day 2
Wed Aug 26
Th Aug 27 |
Nothing Due |
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Day 3
Mon Aug 31
Tu Sept 1 |
Pre-lab 5: Gram stain |
4 points |
Day 4
Wed Sept 2
Th Sept 3 |
Homework #1: Lab Safety and Course Policies; Aseptic technique, Pure culture, Plating, Luminescent bacteria, Microscopy and Staining Pre-lab 7: Enzymes & adaptation |
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| Fri Sept 4 |
Quiz 1 due by noon
Topics:Aseptic technique, Pure culture, Staining, Microscopy |
10 points |
Mon Sept 7
Tu Sept 8 |
Holiday |
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Day 5
Wed Sept 9
Th Sept 10 |
Pre-lab 8: Lactic acid bacteria
Pre-lab 9: Biochemical Tests |
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| Fri Sept 11 |
Quiz 2 due by noon
Topics:Lactic acid bacteria, Enzymes & adaptation |
10 points |
Day 6
Mon Sept 14
Tu Sept 15 |
Homework #2: Enzymes & adaptation, Lactic acid bacteria, Biochemical tests 7: Enzymes & adaptation Worksheet |
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Day 7
Wed Sept 16
Th Sept 17 |
9: Biochemical tests Worksheet |
17 points |
Day 8
Mon Sept 21
Tu Sept 22 |
Pre-lab 11: Snyder test Pre-lab 12: Environmental effects |
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Day 9
Wed Sept 23
Th Sept 24 |
8: Lactic acid bacteria lab report |
24 points |
| Fri Sept 25 |
Quiz 3 due by noon
Topics:Biochemical tests |
10 points |
Day 10
Mon Sept 28
Tu Sept 29 |
10: Growth Curve exercise on LON-CAPA 11: Snyder worksheet Pre-lab 13: Sporeforming bacteria |
32 points
7 points
4 points
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Day 11
Wed Sept 30
Th Oct 1 |
Nothing due |
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| Fri Oct 2 |
Quiz 4 due by noon
Topics:Environmental Effects, Snyder |
10 points |
Day 12
Mon Oct 5
Tu Oct 6 |
Pre-lab 14: Identifictation |
4 points |
Day 13
Wed Oct 7
Th Oct 8 |
Homework #3: Growth Curve, Snyder test, Environmental Effects and Sporeformers |
25 points |
| Fri Oct 9 |
Quiz 5 due by noon
Topics:Sporeformers |
10 points |
Day 14
Mon Oct 12
Tu Oct 13 |
Nothing due |
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Day 15
Wed Oct 14
Th Oct 15 |
Pre-lab 15: Southern Blot |
4 points |
Day 16
Mon Oct 19
Tu Oct 20 |
13: Sporeforming bacteria lab report |
35 points |
Day 17
Wed Oct 21
Th Oct 22 |
Pre-lab 16: Agglutination/ELISA 12: Environmental Effects Formal Lab Report (NO LATE LAB REPORTS WILL BE ACCEPTED)
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Day 18
Mon Oct 26
Tu Oct 27 |
14: Identification worksheet |
21 points |
Day 19
Wed Oct 28
Th Oct 29 |
16: ELISA/Agglutination worksheet |
14 points |
| Fri Oct 30 |
Quiz 6 due by noon
Topics:Identification, ELISA & agglutination |
10 points |
Day 20
Mon Nov 2
Tu Nov 3 |
Pre-lab 17: MIC determination |
4 points |
Day 21
Wed Nov 4
Th Nov 5 |
Pre-lab 18: Transposon mutagenesis conjugation |
4 points |
| Fri Nov 6 |
Quiz 7 due by noon
Topics:Southern Blot |
10 points |
Day 22
Mon Nov 9
Tu Nov 10 |
15: Southern Blot lab report 17: MIC worksheet Pre-lab 19: pGLO |
46 points
9 points 4 points |
Day 23
Wed Nov 11
Th Nov 12 |
Nothing due |
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Day 24
Mon Nov 16
Tu Nov 17 |
19: pGLO worksheet
Pre-lab 20: Analysis of sore throat |
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Day 25
Wed Nov 18
Th Nov 19 |
Homework #4: Southern Blot, pGLO, ELISA & aggluntination, Sore throat, MIC, Transposon mutagenesis |
25 points |
| Fri Nov 20 |
Quiz 8 due by noon
Topics:Transposon Mutagenesis, MIC |
10 points |
Mon Nov 23 to
Fri Nov 27 |
THANKSGIVING BREAK - NO CLASSES |
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Day 26
Mon Nov 30
Tu Dec 1 |
Nothing due |
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Day 27
Wed Dec 2
Th Dec 3 |
18: Transposon Mutagenesis Lab Report
20: Analysis of a sore throat worksheet
Lab Practical Exam, Part I
Dilution problem calculation, Gram stain, Brightfield microscopy, streak plate |
37 points
10 points Parts 1 and 2 worth a total of 100 points |
Day 28
Mon Dec 7
Tu Dec 8 |
Lab Practical Exam, Part II
Dilutions & spread plate, wet mount & phase contrast, biochemical tests, dichotomous key |
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