MCB 150 Frequently Asked Questions

Questions in Transcription and RNA Processing

Page 3 out of 13 Pages

• Why is the stem loop during termination of transcription in E. coli rich in GC?
  
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• On one of our lecture slides, there is a question. Whether human, worm, or fly, the coding region of a gene is the same size. So, what is the answer? Is it NO? I am a little bit confused.
  
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• Among the functions of the Poly(A) tail, I don't understand how it aids in allowing multiple proteins to be efficiently made from a single eukarytotic mRNA.
  
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• In one of our lecture slides, there is a sequence of AAUAA before AAAAAAA. Is this sequence a part of Pre-mRNA or post-transcriptionally added?
  
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• In transcription in E. coli, are the end results the original double stranded DNA and an RNA that double stranded onto itself? If so, is the RNA an exact copy of the DNA?
  
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• Does transcription entail two different things, chromosome transcription and gene transcription?
  
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• During transcription, what does the coding strand do? How is it "similar" to RNA?
  
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• Are the introns being spliced made up of DNA or RNA? i know that they are initially regions of DNA which do not code for RNA..but what happens after transcription?
  
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• Are the conserved sequences at the ends of mRNA that denote splicing sites considered non-translated RNA?
  
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• What is the most abundant form of RNA?
  
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