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Questions in Genetic Engineering and Recombinant DNA Technology
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- Just to clarify, we take our source DNA, so the main genome of the bacterial cell which contains the gene of interest, and we cut it up to get out the gene of interest and that gene is inserted into a vector, such as a plasmid or a phage, and then the vector is the one that replicates the DNA?(20193 views)
- I am unclear on how we check if a CELL has a VECTOR (Selection Step). I understand the Screening step where we can see if a vector has an insert, but not the Selection step.(18359 views)
- I had a simple question regarding the insertion of DNA into the vector. I know that genomic DNA is inserted into the vector, but I am a bit confused. I thought that the restriction enzymes were not allowed to cut the host DNA. Isn't it the host DNA that is inserted to the vector though?(21683 views)
- Aren't restriction enzymes also used to cut viruses that enter? What is that nucleic acid then used for?(24376 views)
- I have written that the 3 ways to introduce the vector into the host are transformation, transduction, and conjugation. Does both conjugation and transformation relate to how a plasmid vector can enter?(23384 views)
- What is the purpose and role of the reporter in a vector?(18965 views)
- Why do introns have to be removed in eukaryotes in order to make a genomic library for producing protein?(18995 views)
- I have a question about gene cloning. If different parts of genomic fragments are used for each of the genes for cloning, how would they be cloning since each of them will have different information in it, and leading them to be different...material? I guess?(18830 views)
- I am really confused about the second step of finding the cell clones that are carrying the insert we're looking for (aka screening). What's its purpose?(17729 views)
- I was just wondering if we are going to have to specifically know any recognition sequences of restriction enzymes. For example, do we need to know that recognition sequence of EcoRI is 5'-GAATTC-3' and others like BamHI and PstI?(18137 views)