MCB 150 Frequently Asked Questions

Questions in Transcription and RNA Processing

Page 7 out of 13 Pages

• Could you hypothetically create two RNA strands from the same gene in parallel by starting a second RNA polymerase on a gene before the first polymerase has finished transcription? If transcription speed is at a premium, this would allow cells to double (or triple, or otherwise increase) their transcription rates. On the other hand, it seems as though there are lots of chances for something to go wrong in this process, such as physical or chemical interactions between polymerases or unfinished RNA strands.
  
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• Do promoters and Hot Spots serve the same purpose with regards to the process of RNA replication?
  
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• When the amino acid becomes attached to the attachment site of the tRNA, what happens at the biochemical level?
  
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• Is the coding strand is the same as the non-template strand?
  
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• Is RNA polymerase of E.coli made of 6 subunits called holoenzyme, then does that mean RNA polymerase IS holoenzyme? if so, why does it have two names?
  
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• what the word Rho mean? Also, are intrinsic and rho-dependent termination the same thing just with different names?
  
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• If transcription terminates by using the Rho method, does the new RNA strand still form a hairpin structure?
  
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• The Poly(A) tail and the 5' cap provide protection from RNase degradation. What is the RNase trying to do that they structures help prevent?
  
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• Are ATP and GTP ever used in transcription?
  
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• How do we differentiate between sequences that sigma subunits are more likely to bind to or less likely to bind to?
  
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