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Questions in Genetic Regulation and the Lactose Operon
Page 1 out of 5 Pages
- I have a question regarding the graph showing glucose and beta-galactosidase concentration vs. cell growth. Why is it that there is no increase of glucose shown in the cell as lactose is broken down into glucose and galactose?(18261 views)
- I had a question regarding the first lecture on genetic regulation. On the bottom of the first page of handouts, I have written 20 AA/sec * 50 sec = 120 ATP per second. I don't understand where the 50 comes from. I would think that you would multiply the 20 AA/sec by the 6 ATPs used per amino acid to get the 120.(19808 views)
- I have written that the protein product of the lacl gene binds to the operator and blocks the promoter and under that I wrote constitutively. Does the constitutively just refer to the fact that the Lac repressor protein is always made even though whether or not it is used depends on the presence of lactose in the absence of glucose?(16340 views)
- I have getting rid of negative regulation is not the same as positive regulation. I was wondering if I'm correct in saying that lactose gets rid of negative regulation, while high levels of CRP (or low levels of glucose) is positive regulation. Help me where I'm wrong...(19485 views)
- Is there any way we can view the animation shown just before you went into the lac operon tutorial?(17111 views)
- Could you explain to me how a bacterial cell regulates the rate of translation. In the notes, it discusses that the cell can control particular tRNA species. How so?(19157 views)
- In bacterial genetic regulation you mentioned that lac mRNA is degraded from the 3' to 5' end, which results in different amounts of protein products being made by the lacZ, lacY, and lacA genes. My question is while the mRNA is being degraded, is there some mechanism that prevents ribosomes from translating the lacA gene entirely, or are ribosomes still translating, but nonfunctional protein is coming out due to the degradation at the 3' end?(15484 views)
- Do Feedback Inhibition and Feedback Control essentially mean the same thing?(19012 views)
- I am confused on the slide about how much it costs to make a protein. Am I right if I state that if you have a gene (lacZ) that is 3,000 nucleotides long, it will uses up (3000 ATP) and give you a mRNA , so in this case, you use 3 ATP per amino acid and therefore if you use 3000ATP, you will have a 1000 AA protein. I'm confused at the part from 3000 nucleotides you get 3000 ATP in the slide, because if you had given us just a 3000 nucleotide gene, then how do I know it uses 3000ATP to make a protein?(18190 views)
- Would the following statement be true or false? "In the absence of lactose, no beta-galactosidase is expressed." I thought that in the absense of lactose the lac repressor protein stops beta-galactosidase from being expressed, so shouldn't this statement be true? If you could clear this up for me I would appreciate it.(17750 views)