ID #1834

Why are there so many more errors in DNA replication when done in vitro?

In vitro experiments only include the "pieces" you choose to include as the researcher doing the experiment. So an in vitro experiment studying DNA replication probably isn't going to include any of the mismatch repair system, which makes your error frequency increase by a factor of 100. And there is a fragment of DNA polymerase that can still do synthesis but cannot do proofreading. Using this in an in vitro experiment would also make your error frequency increase by an additional factor of 100. So it's not the fact that it's being done in vitro that is as important as the fact that your error correction mechanisms are missing!

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