Genetic Engineering and Recombinant DNA Technology

ID #1516

The Reporter gene's purpose within the plasmid vector is to screen for the insert. Also, a common reporter gene is Lac Z. To clarify, is the insert the gene of interest? And, the vector is the means of replicating that gene of interest. And why do we want the reporter gene turned off?


Yes, although it doesn't always have to be an intact gene. Sometimes it's just a piece of DNA that we want to study.

 

And, the vector is the means of replicating that gene of interest. And why do we want the reporter gene turned off? 

 

The inactivation of the reporter gene indicates that something has been inserted into it. That's our DNA of interest! If the reporter gene is still active, it indicates that there's no insert in our vector molecule, since that restriction site is the only place for the insert to go.

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