DNA Structure and Replication

ID #1710

What exactly does it mean that a fragment has been primed? Does it just mean that the enzyme primase has attached to the single-stranded DNA and formed a primer?

No, when a fragment is said to have been "primed," it just means that there is now an available 3' OH group to covalently link DNA nucleotides to. In theory, it could have been primed with RNA or DNA, or with a single base or a thousand. But in E. coli, it will be primed with a small number of RNA bases.

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