DNA Structure and Replication

ID #1115

At the end of one of our lectures, we talked about how certain factors affect the melting temperature that denatures DNA. We said that if there are more GC pairs then it's more stable and harder to disrupt so you would need a higher melting temperature. Then another point is the salt concentration, however I did not catch how that affects the melting temperature. I don't quite understand how the presence of salts would change the melting temperature.

Anything you do to stabilize a double helix means it will take more energy to separate the strands. Since DNA has negative charges lining its backbone (the phosphate groups), and they don't necessarily like being that close to each other, if you neutralize those negative charges, you stabilize the double helix.

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