Energy and Metabolism

ID #1661

Can you please explain why, in substrate-level phosphorylation, the ATP will contain more energy than the original molecule from which the phosphate will be removed?

It's all a question of delta Gs. Just because one molecule loses a phosphate and another gains one does not mean that the energies required for both parts of the reaction are equal. If the delta G for the dephosphorylation event is greater in magnitude than the delta G for the phosphorylation of ADP into ATP, then the substrate level phosphorylation event will proceed.

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