Genetic Engineering and Recombinant DNA Technology

ID #1470

I was just wondering if we are going to have to specifically know any recognition sequences of restriction enzymes. For example, do we need to know that recognition sequence of EcoRI is 5'-GAATTC-3' and others like BamHI and PstI?

Absolutely not. You should be able to identify a likely restriction sequence in a fragment of DNA, but I wouldn't ask you to tell me what enzyme would recognize that sequence.

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