Genetic Engineering and Recombinant DNA Technology

ID #1456

Just to clarify, we take our source DNA, so the main genome of the bacterial cell which contains the gene of interest, and we cut it up to get out the gene of interest and that gene is inserted into a vector, such as a plasmid or a phage, and then the vector is the one that replicates the DNA?


Yes, this is correct, although technically the vector gets replicated, not does the replicating.  But your gene of interest is now part of the recombinant vector molecule and gets copied along with the rest of the vector when the host cell provides the replication machinery.

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