The Nucleus and its Functional Domains

ID #2250

In Lecture 21, Prof. Mehrtens talked about how dTTPs are used as the fluorescent molecule when finding out if DNA is organized. Why does the fluorescence only show up at the ori and not everywhere there's a thymine?


This is because the fluorescent dTTPs are added just before DNA replication. Because DNA synthesis starts at the ori, all fluorescent dTTPs are incorporated there first. If there is an unlimited amount of fluorescent dTTP, the labeling will continue throughout the entire genome. If there is only a finite amount of fluorescent dTTPs, when they run out, the labeling will stop. This can result in fluorescence around the ori only. 

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