ID #1749

When charging the tRNA with an amino acid, does the aminoacyl tRNA synthetase read the anticodon 5' to 3' or 3' to 5'?

Since there is no complementary base pairing going on in this charging event, it's not really "reading" in either direction. It's just a recognition of a specific sequence, and all tRNAs will be held by aminoacyl tRNA synthetases the same way, so those sequences will always be recognized in the same orientation.

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