Translation

ID #2411

For prokaryotes, the small ribosomal subunit will scan along the mRNA until it finds the RBS. Once it finds the RBS, it will place the initiator tRNA. Then the large subunit attaches itself with the initiator tRNA in the p site. Is this correct? It's pretty much the same for eukaryotes right? It will just scan until it finds the first AUG then place the initiator tRNA?


Yes, that basically describes the process in prokaryotes. A few clarifications, though: 
- Once the small subunit finds the RBS, it must find the nearby initiator codon. 
- In all, fMet-tRNA, GTP, and the initiation factors are needed to start the process. Just the fMet-charged tRNA is not sufficient. 
In eukaryotes, the small ribosomal subunit will look for the 5' methylguanosine cap rather than the RBS. 
Eukaryotes use met-tRNA as the first tRNA, rather than fMet-tRNA. 
Also, remember that the subunit sizes are different between eukaryotes and prokaryotes. 

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