Transcription and RNA Processing

ID #1158

In RNA transcription, how far away are from the initiation sites are the promoter regions? I thought it was at -10, which was the TATA box and at -35, but my notes are confusing me. Also am I correct in saying that this only applies to prokaryotes? Do we need to know the location of the promoter region for eukaryotes?

The answer to your first question is that it depends on the RNA polymerase. Also, you should be aware of the fact that a promoter as a whole is a much larger stretch of DNA than just the hot spots. The conserved regions within a bacterial promoter do indeed lie approximately 10 and 35 bases upstream of the +1 base (the first nucleotide in the RNA). And I clearly said in class that you don't have to know the spacing of the conserved regions of eukaryotic promoter sequences.

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