Integrative Biology 474a
Lectures
| Lecture | Date | Topic |
|---|---|---|
Lecture 1 | Aug 23 | Introduction and Overview |
| Lecture 2 | Aug 25 | Basics of 2-Dimensional Electrophoresis and Mass Spectrometry Sample preparation (fractionation) Electrophoresis Bottom-up analysis (peptide mass fingerprinting) |
| Lecture 3 | Aug 30 (Prof. Peter Yau) | Quantitative proteomics—UIUC Proteomics Facility Difference Gel Electrophoresis (DIGE) Isotope-Coded Affinity Tags (ICAT) |
| Lecture 4 | Sept 1 (Prof. Peter Yau) | Proteomics Facility tour and observation—meet in 307 Noyes |
| Lecture 5 | Sept 8 (Prof. Peter Yau) | |
| Lecture 6 | Sept 13 |
Post-translational modifications: Part I |
| Lecture 7 | Sept 15 | PTMs continued O-Glycosylation Redox regulation (-SH) Oxidation |
| Lectures 8-9 | Sept 20 and 22 |
Phosphoserine-binding proteins |
| Lecture 10 | Sept 27 | Phosphoproteome and Protein-Protein Interactions Reading Assignment for Sept 27 Proteomes of organs and subcell compartments |
| Lecture 11 | Sept 29 | Proteomics |
| Lecture 12 | Oct 4 | Proteomics |
| Lecture 13 | Oct 6 | Proteomics |
| Lecture 14 | Oct 11 | Other—Hot topics; Miscellaneous; student presentations (?) |
| Lecture 15 | Oct 13 | In-class FINAL EXAM |


