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Integrative Biology 474a

Lectures

 

LectureDateTopic

Lecture 1

Aug 23

Introduction and Overview
Definition of proteomics
Why study proteomics

Reading for next class

Lecture 2

Aug 25

Pre-lecture slides

Basics of 2-Dimensional Electrophoresis and Mass Spectrometry
Sample preparation (fractionation)
Electrophoresis
Bottom-up analysis (peptide mass fingerprinting)
Lecture 3Aug 30
(Prof. Peter Yau)
Quantitative proteomics—UIUC Proteomics Facility
Difference Gel Electrophoresis (DIGE)
Isotope-Coded Affinity Tags (ICAT)
Lecture 4Sept 1
(Prof. Peter Yau)
Proteomics Facility tour and observation—meet in 307 Noyes
Lecture 5Sept 8
(Prof. Peter Yau)

Top-Down Mass Spectrometry and tour of IGB mass spec lab

Required Reading

Lecture 6Sept 13

Basics continued

Post-translational modifications: Part I
Protein Phosphorylation

Lecture 7Sept 15PTMs continued
O-Glycosylation
Redox regulation (-SH)
Oxidation
Lectures 8-9 Sept 20
and 22

Lecture 8 slides

Lecture 9 slides

Phosphoserine-binding proteins
Protein chips and arrays
Protein complexes

Required Reading for Sept 20

Homework II due Sept 22

Peaklist for Homework II

Lecture 10Sept 27

Phosphoproteome and Protein-Protein Interactions

Lecture 10 slides

Reading Assignment for Sept 27

Proteomes of organs and subcell compartments
Arabidopsis ecotypes
Leaf (chloroplast; thylakoid; mitochondria); cell wall; apoplast; Seed

Lecture 11Sept 29 Proteomics
Lecture 12Oct 4 Proteomics
Lecture 13Oct 6 Proteomics
Lecture 14Oct 11Other—Hot topics; Miscellaneous; student presentations (?)
Lecture 15Oct 13In-class FINAL EXAM