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Integrative Biology 472

Plant Molecular Biology


Although "omics" approaches are revolutionizing basic and applied plant biological research, understanding the mechanisms by which gene expression is regulated and how gene products function often requires a one-gene-at-a-time (OGAAT) approach. This course provides an overview of selected topics that fall under the OGAAT approach. It will cover basic measurements of gene expression, mechanisms regulating transcription, and the construction of transgenic plants for addressing specific biological questions. This course will revolve around understanding experimental techniques and how they can be applied to understanding mechanisms that regulate gene expression.


This course is aimed at developing students' abilities to read and interpret OGAAT literature, to develop insights into the strengths and weaknesses of this approach, and to apply this approach in their own research when suitable.


Place and time:

132 Davenport Hall, 10-10:50 am, Monday and Wednesday, Aug. 23 Oct. 15, 2010


Optional textbooks:

If you would like a text as a general reference, consider either Molecular Biology of the Cell, 4th ed., B. Alberts, et al. (2002) or for a simpler and more general text that covers more about the mechanics of recombinant DNA technology, try Recombinant DNA: Genes and Genomics: A Short Course (Paperback), 3rd ed., J. Watson et al. (2006). Both of these are available (new and used at "reasonable" prices) at


PDF files of recommended reading materials will be posted on Compass.



Ray Ming, 148 ERML

Office hours: after class and by appointment

Phone: 333-1221; Email: