Integrative Biology 335 —
Methods and Principles of Systematics

Announcements:

Lecture Assignment 1 is due in class today (Wednesday)! Late assignments (anytime after 10 AM on due days) will receive a 50% penalty. Assignments more than 24 hours late will NOT be accepted. A link to answers for this assignment will be posted on the lecture syllabus after class today. Corrected assignments will be returned in lab.

Graduate students wanting to take this course for IB 490 credit, please send me an email to arrange a meeting ASAP!

Cladogram and classification for Lecture Quiz 4 Remember, to underline Latin names (including subgeneric names). For full points, you must show the positions of all synapomorphies on the phylogeny!


Readings:

Textbook by Judd et al., Plant Systematics: A Phylogenetic Approach, 3rd edition

This is required reading. However, there is some irrelevant information in these chapters (or material we won't cover until later in the course), so only read and learn that information that is relevant to addressing today's objectives.

Web Resources:


General Objectives:

After studying this material you should be able to:

  1. Describe what a phylogeny is and how one can be constructed or interpreted using the method of parsimony. Given a simple data set, you should be able to construct a cladogram.
  2. Have a basic knowledge of the relationships among individuals, populations, species, and the phylogenetic tree.
  3. Explain the major stages or changes that have to occur before a new species can arise.
  4. Define some important terms of phylogenetic methodology, such as monophyly, synapomorphy, parsimony, homoplasy, polyphyly, and paraphyly.
  5. Explain why "dicots" are rejected as a formal group in phylogenetic classifications.
  6. Describe how a phylogeny can be translated into a phylogenetic classification.
  7. Start becoming familiar with the relationships among, and names of, the major clades recognized by the Angiosperm Phylogeny Group (i.e., magnoliids, monocots, eudicots (or tricolpates), basal tricolpates, core eudicots, rosids, eurosids I (or fabids), eurosids II (or malvids), asterids, euasterids I (or lamiids), euasterids II (campanulids), and "basal angiosperms" - the ANITA grade).


Review: Approaches to Classification

The characters which naturalists consider as showing true affinity between any two or more species, are those which have been inherited from a common parent, all true classification being genealogical.
Charles Darwin 1859: 391

Happy Birthday, Darwin! Feb. 12 is Darwin's birthday. This year marks the 200th anniversary of Charles Darwin's birth. The year 2009 is also the 150th anniversity of the publication of his fundamental work, On the Origin of Species.

Crunching the Data for the Tree of Life, The New York Times Science article (February 10, 2009)

Classification: the theory and practice of grouping and ranking organisms.

There are many ways to do this -- and you will learn about some of these below and in a later lecture on historical systematics -- but the approach we and your textbooks take is phylogenetic. There are two major steps in producing a phylogenetic classification:


What is a Phylogeny?

A phylogeny is a diagram (a phylogenetic tree or cladogram) that depicts the evolutionary relationships among organisms. Comparative morphological, anatomical, embryological, molecular, behavioral, physiological, chemical, geographical, and fossil data can all be used, together or separately, to construct the phylogeny.

A phylogeny provides the historical perspective from which to interpret the evolution of characters, patterns and processes of diversification, rates of evolution, historical biogeography, and co-evolutionary phenomena, such as the relationships between hosts and parasites or between plants and their insect herbivores.

A phylogeny is used to classify organisms on the basis of their inferred evolutionary relationships (the phylogenetic approach to classification).

A phylogeny is a scientific hypothesis based on the best interpretation of the data at hand and subject to further evaluation (and possibly change) as new data become available.


Introduction to the Concept of Phylogeny

Levels of detail in genetic history: from individuals to the phylogenetic tree.


How Do New Species Arise?


The Divergence of Populations

Stages in the formation of a new species (from Grant, 1963 and 1981, and the University of Alabama). This is basically the same illustration as provided below, with more detail.

Here is an illustrative example of geographic speciation from the University of Alabama. This poor quality figure shows the separation of two populations by some geographic barrier.

If a population should become divided into two by a geographic barrier (or if some individuals are transported to a new area outside the parent's range), evolution of each new population continues independently due to the forces of natural selection, genetic drift, migration, nonrandom mating, and mutation. Differences between the two, including differences in reproductive processes, gradually accumulate such that reproductive isolating mechanisms become more and more effective over prolonged periods of time.

New species arise when genetic differences accumulate to the point when the two groups can no longer successfully mate and reproduce (if and when they come back into contact). Species can be defined as groups of actually or potentially interbreeding populations that are reproductively isolated from other such groups.

Speciation events lead to the multiplication and diversification of species into higher taxa (e.g., genera, families, orders, classes, phyla, etc.). All species (animals, plants, fungi, and all major groups of microorganisms) can be traced back to a single origin of life on earth. Evolution is a continuing process that explains the history of life on earth, as well as the diversity of life today.


Geographic isolation is NOT always necessary for speciation to occur

Speciation can occur within the range of the parent population (and sometimes quite rapidly). Gene flow can be disrupted by:


The splitting of populations and evolutionary modifications

The illustration below shows the splitting of populations into separate lineages (each circle represents a plant). Members of the new populations have new gene characteristics and, possibly, changes in their overall form.

Below, in a very simple example, white-flowered ancestors give rise to descendants having either red or blue flowers. Each of these new lineages then acquire further distinctive characteristics. These new (or derived) features (relative to those found in the ancestors) tell us that a new lineage has been established.

Summarization

The diagram above can be summarized as branching trees. Only those features that have changed (the derived characteristics) are indicated on the bottom tree.


The phylogenetic approach to classification

Dissatisfied with both the phyletic or natural approach (because of its intuitive nature) and with phenetics (because it reflects total similarity and not evolutionary history), taxonomists have sought an explicit approach to classification that directly reflects evolutionary relationships. Phylogeneticists work under the principle that there is a single and historically unique genealogic history relating all organisms.

Phylogenetics or cladistics is a philosophical and methodological approach to classification that attempts to recover genealogical relationships among groups of organisms by producing branching trees (called phylogenies or cladograms) that reflect these relationships. Its core concept is the use of shared derived character states to reconstruct common ancestry. This concept was first formalized by Willi Hennig, a German entomologist, in a landmark book published by the University of Illinois Press. Hennig argued that the best classification of organisms is one that exactly reflects the genealogical relationships among these organisms.

Some definitions:


Determining Evolutionary History

More definitions:

There are other terms that are defined in your textbook, but for now, we will not hold you responsible for knowing them.


Methodology

Make evolutionary assumptions. This includes selecting the taxa to be examined and determining whether they are monophyletic or not. (Often, this isn't confirmed until the study is complete.)

Select characters of evolutionary interest. In practice, the number of characters much be one less than the number of taxa for all branches of the tree to be resolved. The characters used can be diverse, representing those obtained from both morphology and molecular studies. In phenetic approaches to classification, many more characters are used.

Describe and/or measure the character states. It is essential to ensure that the same kinds of characters are being compared from one species to another. (One state of a character is derived directly from another state of that same character.)


Constructing a data matrix

Once the characters have been selected, an appropriate coding (using letters or numbers) must be assigned to the states so that they can be placed conveniently into a data matrix. In the matrix below, the number "1" is assigned to those character states that represent the derived condition and the number "0" is assigned to those character states representing the ancestral condition. (This isn't always so, however. Often, the "polarity" of a character state isn't known until after the analysis is done.)

The most commonly used method for constructing cladograms is the method of parsimony. This method attempts to minimize the number of character state changes among the taxa (the simplest evolutionary hypothesis). Using shared derived character states, the trees are constructed. (There are other methods of tree construction that can also be used, such as minimum distances.)

Interpretation:


Phylogenetic Classifications

Integrating the results of a phylogenetic analysis into a classification is an extremely contentious issue in systematics. Basically, taxa are recognized on the basis of monophyly. Then the groups are ranked and placed in a hierarchy. In the cladogram above, for example, species A, B, C & D may all be recognized as comprising the same genus (with two subgroups, possibly) and species E & F as comprising another genus. Or, species A-D may comprise one family (with two subfamilies, possibly) and species E & F another family.

BUT

species E, F & D should not be recognized as a taxon (upon the exclusion of species A, B & C) nor should species A, B & C be recognized as a taxon (upon the exclusion of species D). Each of these two taxa are not monophyletic because they each do not contain all the descendants of their common ancestors.

It's important to realize that many current classifications do NOT represent phylogenies. They were developed many years ago, before anyone had any idea about evolution.


Monophyly, Polyphyly, and Paraphyly

This information comes from Wendy B. Zomlefer, Guide to Flowering Plant Families, The University of North Carolina Press, p. 15.


"Dicotyledons" and Monocotyledons: an example of paraphyly

The "dicots" do not form a monophyletic group (they do not contain all the descendants of their common ancestor). They are rejected as a formal group. Basically, it's difficult to circumscribe the "dicots" without including the monocots. Some "dicots" are more similar to monocots than they are to other "dicots." The "dicots" are paraphyletic, whereas the monocots can be defined by several synapomorphies.

Within angiosperms, three main lineages are apparent: a Magnolia-like group (e.g., Magnoliaceae), a tricolpate-pollen group (e.g., the typical "dicots"), and a paleoherb group (which contains the monocots). This cladogram may more accurately reflect angiosperm relationships than the conventional monocot-dicot split.


Angiosperm Phylogeny

The relationships among the major angiosperm groups are modeled after the system of the Angiosperm Phylogeny Group 2003 (referred to as APG II, 2003; Botanical Journal of the Linnean Society, 2003, 141: 399-436). This system is based on published cladistic analyses primarily using molecular data. Only those families that are monophyletic are recognized. The APG II system classifies families into orders, where strong evidence suggests that the order is monophyletic. Some monophyletic groups containing several orders are given informal names, such as "magnoliids," "monocots," "eudicots," etc. As much as we are able, we will follow the APG II classification in IB 335. This phylogeny is the same as the one used in the Judd et al. textbook.

The more inclusive categories of the taxonomic hierarchy that we will be studying this semester include:


Lecture Assignment Two

Lecture Assignment Two will be handed out in class today. Click here to learn more about doing this assignment.


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