Biophysics 354, Fall 2000
Meeting: 423, Natural History Building, 2.00 - 3.30 p.m., Tuesdays and Thursdays
Instructor: A. R. Crofts, 149 Davenport Hall (a-crofts@uiuc.edu)
Course assistance: Dr. Vlad Shinkarev, (vshinkar@uiuc.edu)
Office hours: Friday, 2-4:00 pm, 144 Davenport Hall
| Date | Topic | Physical chemistry discussed | Hours |
| 8/24 | Introduction | Biological energy conversion,
overview and global perspective. |
1.5 |
| 8/29 8/31 | Cellular Metabolism
Review of glycolysis;
|
Thermodynamic functions; equilibrium constant,
pH, pK, enthalpy, entropy and free energy. |
3 |
| 9/5 9/7 | Review of aerobic metabolism;
Review of TCC; substrate level phosphorylation |
Coupling between reactions; DGo'
for ATP
hydrolysis; redox reactions; electrode potential and relation to free energy. |
3 |
| 9/12 9/14 | Aerobic Bioenergetics | Effects of ligands, H+, on redox
potential. Redox potentiometry. |
3 |
| 9/19 9/21 | Electron transport chains
as proton pump |
Chemical potential; electrochemical
potential; membrane potential; coupling between electron and proton transport. |
3 |
| 9/26 9/28 | ATP synthesis | The proton circuit, surface potential,
local proton activity; mechanisms. |
3 |
| 10/2 10/5 | Proton conduction, stoichiometry | Methodology; spectrophotometry; selective electrodes.
Work, power, and potential. |
3 |
| 10/10 10/12 | Transport and Motility | Kinetics and thermodynamics of transport. | 3 |
| 10/17 10/19 | Motility, muscle and
related systems |
Chemical and mechanical work. | 3 |
| 10/24 10/26 | Electron transfer; kinetic aspects | 1st and 2nd order kinetics, relation to equilibrium constant;
activation energy, theory of electron transfer. |
3 | 10/31 11/2 | Solar Energy Conversion | Energetics of exciton transfer;
fluorescence; Forster theory. |
3 |
| 11/7 | MID TERM EXAM | (Take home exam, no class this day) | 1.5 |
| 11/9 11/14 | Photochemical reaction centers, structures: bacterial RC, photosystem II |
Phototrapping; kinetics and
thermodynamics; mechanism |
3 |
| 11/16 11/21 | Photosystem II; oxygen evolution; two-electron gates | O2 evolution, detailed discussion of mechanism | 3 |
| 11/23 | Thanksgiving break | (No class this day) | |
| 11/28 11/30 | Photosystem I, and electron transfer between and around the photosystems | Photosynthetic electron transfer Intermediate chains bc1 complex; and b6f complex Couping to metabolism |
3 |
| 12/5 12/7 | Coupling to the proton gradient: Membrane voltmeters; delayed fluorescence; protolytic reactions |
Electrochromism; rapid pH changes. Coupling in the steady state | 3 |
| 12/9 | Reading day | (No class this day) | 1.5 |
| 12/14 | FINAL EXAM DUE, 6.30 p.m. | (TAKE HOME EXAM replaces scheduled exam, 7:00-10:00 PM, Thur, Dec 14) |
Textbook
The WWW material for this course is fairly comprehensive. If you feel you need a textbook, the following is recommended:
Authors: W.A. Cramer and D.B. Knaff
Title: Energy Transduction in Biological Membranes: A Textbook of Bioenergetics
Publisher: Springer-Verlag New York/Berlin/London
Recommended
(You should have a Physical Chemistry textbook available for reference, but any good text will be sufficient).
Authors: Tinoco, Sauer and Wang
Title: Physical Chemistry, Principles and Applications in the Biological Sciences
Publishers: Prentice Hall
Author: David Walker
Title: Energy, Plants and Man
Publishers: University Science Books, Mill Valley, CA 94941