Biophysics 354, Fall 2000
Meeting: 423, Natural History Building, 2.00 - 3.30 p.m., Tuesdays and Thursdays
Instructor: A. R. Crofts, 149 Davenport Hall (firstname.lastname@example.org)
Course assistance: Dr. Vlad Shinkarev, (email@example.com)
Office hours: Friday, 2-4:00 pm, 144 Davenport Hall
|Date||Topic||Physical chemistry discussed||Hours|
|8/24||Introduction||Biological energy conversion,
overview and global perspective.
|8/29 8/31||Cellular Metabolism
Review of glycolysis;
|Thermodynamic functions; equilibrium constant,
pH, pK, enthalpy, entropy and free energy.
|9/5 9/7||Review of aerobic metabolism;
Review of TCC; substrate
|Coupling between reactions; DGo'
hydrolysis; redox reactions; electrode
potential and relation to free energy.
|9/12 9/14||Aerobic Bioenergetics||Effects of ligands, H+, on redox
potential. Redox potentiometry.
|9/19 9/21||Electron transport chains
as proton pump
|Chemical potential; electrochemical
potential; membrane potential; coupling
between electron and proton transport.
|9/26 9/28||ATP synthesis||The proton circuit, surface potential,
local proton activity; mechanisms.
|10/2 10/5||Proton conduction, stoichiometry||Methodology; spectrophotometry; selective electrodes.
Work, power, and potential.
|10/10 10/12||Transport and Motility||Kinetics and thermodynamics of transport.||3|
|10/17 10/19||Motility, muscle and
|Chemical and mechanical work.||3|
|10/24 10/26||Electron transfer; kinetic aspects||1st and 2nd order kinetics, relation to equilibrium constant;
activation energy, theory of electron transfer.
|3||10/31 11/2||Solar Energy Conversion||Energetics of exciton transfer;
fluorescence; Forster theory.
|11/7||MID TERM EXAM||(Take home exam, no class this day)||1.5|
|11/9 11/14||Photochemical reaction centers,
structures: bacterial RC, photosystem II
|Phototrapping; kinetics and
|11/16 11/21||Photosystem II; oxygen evolution; two-electron gates||O2 evolution, detailed discussion of mechanism||3|
|11/23||Thanksgiving break||(No class this day)|
|11/28 11/30||Photosystem I, and electron transfer between and around the photosystems||Photosynthetic electron transfer
bc1 complex; and b6f complex
Couping to metabolism
|12/5 12/7||Coupling to the proton gradient:
Membrane voltmeters; delayed fluorescence; protolytic reactions
|Electrochromism; rapid pH changes. Coupling in the steady state||3|
|12/9||Reading day||(No class this day)||1.5|
|12/14||FINAL EXAM DUE, 6.30 p.m.||(TAKE HOME EXAM replaces scheduled exam, 7:00-10:00 PM, Thur, Dec 14)|
The WWW material for this course is fairly comprehensive. If you feel you need a textbook, the following is recommended:
Authors: W.A. Cramer and D.B. Knaff
Title: Energy Transduction in Biological Membranes: A Textbook of Bioenergetics
Publisher: Springer-Verlag New York/Berlin/London
(You should have a Physical Chemistry textbook available for reference, but any good text will be sufficient).
Authors: Tinoco, Sauer and Wang
Title: Physical Chemistry, Principles and Applications in the Biological Sciences
Publishers: Prentice Hall
Author: David Walker
Title: Energy, Plants and Man
Publishers: University Science Books, Mill Valley, CA 94941