Single mutations in cytochrome b

Click here for Table 1b, Multiple mutations


Table 1a.  Single mutations in the cyt b subunit of the bc1 complex from prokaryotes and eukaryotes and their salient effects

#	Residue			Growth Phenotype			Properties 					References
Prokaryotes/Eukaryotes		Prokar./Eukar.		Prokaryotes			/Eukaryotes
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1	I33/I17F		*  /Gly+		*				/DiR, HQR				[5]
2	W45/W30C		*  /Gly+		*				/FuR, HQR				[19]
3	I46/N31K		*  /Gly+		*				/AnR, DiR, HQR				[5]
4	G48D/G33D		Ps-/Gly-	Affects assembly			/Affects assembly			[7,20,21]
	G48V/G33		Ps-/*		Modified bL and bH, Qi-			/*					[7]
	rG48A/rG33A		Ps+/Gly+	No effect				/FuR, HQR, Shifted bH			[7,20,21]
5	A52V/G37V 		Ps+/Gly+	AnR, Affects Qi kinetics		/AnR, FuR, HQR				[7,5]
	A52D/G37V(M. m.) 	Ps-/na		Affects assembly			/AnR, FuR				[4,25]
	A52/A37V,G(S. p.) 	*  /na			*				/AnR, DiR				[4]
6	Q58L/Q43		Ps+/*		Modified bL				/*					[7]
7	H97D,N/H82		Ps-/*		bL and bH absent			/*					[7]
8	S102A/S87		Ps+/*		No effect				/*					[7]
9	F104I/F89		Ps+/*		No effect				/*					[7]
10	L106P/M91(fs)		Ps+/Gly-	MyR, MucR				/nd					[7,22]
11	H111Y,N,D/H96		Ps-/*		bH absent				/*					[7]
12	R114Q/K99		Ps-/*		Affects assembly			/*					[15]
	R114A,R/K99		Ps-/*		Inactive				/*					[23,15]
	R114K/K99		Ps+/*		Lower Em of bH				/*					[15]
13	G119/G104(fs)		*  /Gly-		*				/Affects assembly			[22,24]
	Y118/rY103I		*  /Gly+		*				/nd					[22,24]
14	W129A/W114		Ps+/*		Qi kinetics perturbed			/*					[15]
	W129F/W114		Ps+/*		Low level of bc1			/*					[15]
15	M140G,C/I125		Ps+/*		No effect				/*					[8]
	M140R/I125		Ps-/*		Occupied Qo, QH2 ox-			/*					[8,26]
	M140I,V,W/I125		Ps+/*		MyR, StR				/*					[8]
	M140S/I125		Ps+/*		MyR					/*					[8]
	M140L,N,D/I125		Ps+/*		StHS					/*					[8]
16	G141/A126T		*  /Gly+		*				/MyR, FuR				[6]
17	F144R,K/F129		Ps-/*		Ion pair at Qo, QH2 ox-			/*					[26,8]
	F144S,T/F129		Ps+/*		MyR, Partially occupied Qo,
						Slow QH2 ox				/*					[26,27,8]
	F144V/F129		Ps+/*		MyR, StR, Partially occupied Qo,	/*					[26,27,8]
	F144I,G,L/F129L		Ps+/Gly+	MyR, StR, Empty Qo, Slow QH2 ox		/MyR, StR, MuR, Modified bL 		[26,27,6]
	F144N/F129		Ps+/*		MyR, Empty Qo, Slow QH2 ox, 		/*					[8,27,5]
	F144A,H/F129L(C. r.)	Ps+/na		MyR					/MyR, MuR				[4,8]
	F144Y,W/F129		Ps+/*		StR					/*					[8,26,27]
	F144C/F129		Ps+/*		MyR, StR				/*					[8]
18	G146A,V/G131S		Ps-/Gly-	Modified bL, Empty Qo, QH2 ox-		/Affects assembly			[28,5]
19	Y147F/Y132C(C. r.)	Ps+/na		Slow QH2 ox				/MyR, MuR				[29,4]
	Y147V/Y132		Ps+/*		MyR, Slow QH2 ox			/*					[29]
	Y147A,S/Y132		Ps-/*		Occupied Qo, QH2 ox-			/*					[29]
20	V148/C133Y		*  /Gly-		*				/MyR, Modified bL,
											Qo and Qi red affected			[5,30]
	V148/rC133S,F,D,H	*  /Gly+		*				/nd					[5]
21	G152T,D,E,H,R/G137R	Ps+/Gly+	MyR					/MyR, MuR				[8,5]
	G152K,C,Y,I,F,L,V,N
	/G137S(C. r.)		Ps+/na		StR, MyR				/MyR, MuR				[4,8]
	G152P/G137E		Ps-/Gly-	Affects stability			/StR, MyR, MuR, Uncoupled and 
											affects stability (FeS)		[7,8,13,16,31]
	G152S/G137V		Ps+/Gly-	MyR, MucR				/MyR					[7,8,5]
	G152A/rG137A		Ps+/Gly+	StR					/Slow growth				[7,8,5]
22	Q153/Q138 Stop		*  /Gly-		*				/nd					[5]
	Q153/rQ138Y,E,L		*  /Gly+		*				/nd					[5]
23	M154V/M139		Ps+/*		MyR					/*					[29]
24	S155C/S140		Ps-/*		nd					/*					[32]
25	W157A,S/W142R		Ps+/Gly-	Low (30%) bc1 activity			/MyHS, Qo and Qi red affected		[32,33] 
	W157/rW142K,T		*  /Gly+		*				/MyR, Low bc1 activity			[33]
	W157/rW142S		*  /Gly+		*				/Minor effect				[33]
26	G158A,S/G143A(M. m.)	Ps+/na		MyR					/MyR, MuR	 			[4,8,27]
	G158T,C,N,D,E,H,R,L,I,V,W	
	/G143			Ps-/*		Empty Qo, QH2 ox-			/*					[27]
27	T160F/T145		Ps-/*		Empty Qo, Slow QH2 ox			/*					[8,34]
	T160R,S,C,L,Y/T145	Ps+/*		MyHS, Partially occupied Qo, 
						Low bc1 activity			/*					[3,34]
	T160A,V			Ps+/*		Minor effects				/*					[23]
28	I162G,A,L,F/I147F	Ps+/Gly+	No effect				/StR					[32,5]
29	T163A,C/T148M(M. m.)	Ps+/na		StR					/StR					[8,4]
	T163V,L,I/T148		Ps+/*		MyHS					/*					[8]
	T163R/T148		Ps-/*		Empty Qo site, 5 % bc1 activity		/*					[8,26]
	T163F,P/T148		Ps-/*		Affects assembly			/*					[8]
	T163S/T148		Ps+/*		No effect				/*					[8,14]
30	L165G,A/L150F		Ps+/Gly-	No effect				/nd					[5,32]
31	F166Y,W,L,G,V/F151C	Ps+/Gly-	No effect				/nd					[5,32]
32	G167S/S152		Ps+/*		No effect				/*					[32]
33	P170S/P155		Ps+/*		No effect				/*					[32]
34	G171S/F156		Ps+/*		No effect				/*					[32]
35	S175C/N159		Ps+/*		No effect				/*					[32]
36	G182S/G167		Ps+/*		Slow QH2 ox 				/*					[32]
37	A185C/S170		Ps+/*		No effect				/*					[32]
38	H198N,Y/H183		Ps-/*		bH and bL absent			/*					[7]
39	P202L/P187		Ps+/*		No effect				/*					[7]
40	V209A/V194		Ps+/*		FuS					/*					[23]
	V209T/V194		Ps+/*		No effect				/*					[23]
41	H212D/H197		Ps-/*		bH absent				/*					[7]
	H212Q/H197		Ps-/*		Affects assembly			/*					[23]
42	I213V/L198F		Ps+/Gly+	Qi kinetics perturbed			/FuR					[23,5]
43	H217A/H202		Ps-/*		QH2 ox-					/*					[35]
	H217R/H202		Ps+/*		More stable SQi- at Qi, Qi
						kinetics perturbed			/*					[36]
	H217L/H202		Ps-/*		Unstable SQi- at Qi, Qi-		/*					[36]
	H217D/H202		Ps+/*		Unstable SQi- at Qi, Qi
						kinetics perturbed			/*					[36]
	H217Q/H202		Ps-/*		Affects assembly			/*					[23]
44	S218A/I203		Ps+/*		No effect				/*					[23]
45	T219A/H204(fs)		Ps+/Gly-	Slow QH2 ox, Modified Em of bL	/Affects assembly			[23,37,38]
	T219/rH204Y		*  /Gly+		*				/HQR					[37,38,39]
46	G220V/G205		Ps+/*		Qi kinetics perturbed			/*					[23]
47	N221I/S206L		Ps+/Gly-	Slow bH ox				/HQR, Shifted bH, Qi perturbed 	[5,21,23]
	N221/rS206T,V		*  /Gly+		*				/HQR					[37,38,39]
	N221S/S206		Ps+/*		No effect				/*					[23]
48	N222A/S207		Ps+/*		Qi kinetics perturbed			/*					[23]
49	N223Q/N208		Ps+/*		Em of bH shifted			/*					[23]
	N223V/N208		Ps+/*		Qi kinetics perturbed			/*					[23]
	N223A,D/N208		Ps+/*		No effect				/*					[23]
50	P224A/P209		Ps+/*		No effect				/*					[23]
51	F244L/M221K		Ps-/Gly-	Affects assembly			/An and Fu binding 
												modified, Qi-			[5,21,23,40]
	F244/rM221E,Q		*  /Gly+		*				/DiR, HQR				[20,21]
52	F248L/F225L,S		Ps-/Gly+	Affects assembly			/DiR, HQR				[5,23]
53	K251M/K228M(K. l.)	Ps+/na		AnR, Slow bH to Qi kinetics		/AnR					[4,35]
	K251I/K228I		Ps+/Gly+	AnR, Slow bH to Qi kinetics		/AnR					[5,35]
54	D252A/D229		Ps-/*		AnR,bH ox blocked			/*					[35,25]
	D252N/D229		Ps+/*		AnR, Qi-				/*					[35,25]
	D243H,E(R. r.)/D229	Ps+/*		AnR, Qi site defective			/*					[8,41]
55	A255/G232D(M. m.)	*  /na			*				/AnR, HQR, FuR				[4]
56	F263/L240(fs)		*  /Gly-		*				/nd					[22, 24]
57	N279Y/N256Y 		Ps+/Gly+	StR, MyR				/StR, MyR, MuR, Affects
												stability (FeS)			[5,23]
	N279/N256K		*  /Gly+		*				/MuR, StR				[13]
	N279/N256I		*  /Gly+		*				/MuR, StR, Affects stability (FeS) [13]
58	I292F/I269		Ps-/*		QH2 ox-					/*					[23]
59	P294S/P271		Ps+/*		Slow QH2 ox				/*					[23]
	P294L/P271		Ps-/*		Affects assembly			/*					[23]
60	E295D,G/E272		Ps+/*		StR, Slow QH2 ox			/*					[23]
	E295Q/E272		Ps+/*		StR, QH2 ox-				/*					[23,25]
61	W296L/W273		Ps+/*		StR, MyR, Slow QH2 ox			/*					[23]
	Y297F/Y274		Ps+/*		Slow QH2 ox 				/*					[23]
63	F298/L275F		*  /Gly+		*				/MyR					[5]
	F298/L275S,T		*  /Gly+		*				/MuR,MyR				[5]
64	L305D/L282F		Ps+/Gly-	Slow QH2 ox				/Affects assembly			[5,23]
	L305A/rL282I,L		Ps-/Gly+	Affects assembly			/nd					[23,42]
	L305/rL282C,T		*  /Gly+		*				/Slow growth				[23,42]
65	K329G/K288		Ps+/*		Slow QH2 ox				/*					[23]
	K329A/K288		Ps-/*		Affects assembly			/*					[23]
66	G332S/G291		Ps+/*		Slow QH2 ox				/*					[23]
	G332I/G291		Ps-/*		Qo site blocked				/*					[23]
	G332D/G291		Ps+/*		Slow QH2 ox				/*					[23]
67	V333A,M/V292		Ps+/*		StR					/*					[8,23]
	V333G,R,L/V292		Ps+/*		No effect				/*					[8]
68	M336/L295F(M. m.)	*  /na			*				/MyR, StR				[4]
69	Y358F,T/F318		Ps+/*		No effect				/*					[32]
70	F367L/K326		Ps+/*		No effect				/*					[32]
71	W368R/F327		Ps+/*		No effect				/*					[32]
72	A371Q/V330		Ps+/*		No effect				/*					[32]
73	D373V/N332		Ps+/*		Lower bc1 activity			/*					[32]
74	F374T/F333		Ps+/*		No effect				/*					[32]
75	V376/L335Stop		*  /Gly-		*				/Affects assembly			[43]
76	G381/G340E		*  /Gly-		*				/Affects assembly			[5]
	G381/rDG340		*  /Gly+		*				/nd					[42]
	G381/rG340A,V		*  /Gly+		*				/nd					[42]
77	Y389/Y348(fs)		*  /Gly-		*				/Affects assembly			[43]
	Y389/rY348I		*  /Gly+		*				/nd					[43]
	D390/rV349C		*  /Gly+		*				/nd					[43]