COMPUTER AIDED FLUORESCENCE IMAGING OF PHOTOSYNTHETIC SYSTEMS: Application of video imaging to the study of fluorescence inductionin green plants and photosynthetic bacteria


by James M. Fenton and Antony R. Crofts

Department of Physiology and Biophysics, University of Illinois, 407 S. Goodwin Ave., Urbana, IL 61801

Abstract

A fluorescence video imaging system utilizing relatively inexpensive commercial components is described. The instrument utilizes a black and white CCD video camera detector, a commercial video imaging board and a IBM- AT compatible computer. The color output of the imaging board greatly aids in the users ability to visually discriminate areas of interest in the video field. Software developement that enables the user to capture kinetic traces in real time from the video images is also described. The system is used to monitor fluorescence from photosynthetic systems. The usefulness of the system in screening for photosynthetic mutants is also demonstrated.