Topic Physical chemistry discussed Lecture Hours Cellular Metabolism Introduction Biological energy conversion, 1 overview and global perspective. Review of glyco- Thermodynamic functions; equilibrium 2 lysis; anaerobic constant, pH, pK, enthalpy, entropy and metabolism, etc. free energy. Role of ATP in cell; Coupling between reactions; G'o for ATP 3 Review of TCC;substrate hydrolysis; redox reactions; electrode level phosphorylation. potential and relation to free energy. Aerobic Bioenergetics Electron transport Effects of ligands, H+, on redox 3 chains; components potential. Redox potentiometry. Electron transport Chemical potential; electrochemical 3 chains as proton pumps. potential; membrane potential; coupling between electron and proton transport. ATP synthesis. Proton conduction, surface potential; 3 structure and mechanism of ATP-synthase. Stoichiometry. Methodology; spectrophotometry; select- 3 ive electrodes. Work, power, and potential. MID TERM EXAM (Take home exam, no class this day) 1.5 Transport and Motility Metabolite transport; Kinetics and thermodynamics of 3 bacterial transport. transport. Motility, muscle and Chemical and mechanical work. 3 related systems. Electron Transport and kinetics Electron transfer; 1st and 2nd order kinetics, relation to 3 kinetic aspects equilibrium constant; activation energy, theory of electron transfer. Solar Energy Conversion Light harvesting Energetics of exciton transfer; 3 fluorescence; Forster theory. Photochemical reaction Phototrapping; kinetics and 3 centers thermodynamics; two-electron gate. Photosynthetic electron Detailed discussion of mechanism; 3 transfer bc-complex; oxygen evolution Proton gradient Membrane voltmeters and electrochromism; 3 rapid protolytic reactions; pH changes Coupling in the steady Introduction to non-equilibrium 3 state thermodynamics. FINAL EXAM (TAKE HOME EXAM)
Authors: W.A. Cramer and D.B. Knaff
Title: Energy Transduction in Biological Membranes: A Textbook of Bioenergetics
Publisher: Springer-Verlag New York/Berlin/London
Authors: Tinoco, Sauer and Wang
Title: Physical Chemistry, Principles and Applications in the Biological Sciences.
Publishers: Prentice Hall
(You should have a Physical Chemistry textbook available for reference; the above is recommended as providing a useful biological perspective, but any good text will be sufficient).
Author: David Walker
Title: Energy, Plants and Man
Publishers: University Science Books, Mill Valley, CA 94941