Rice Microarray Information

Printing Details

• Print on ArrayIt SuperAmine slides (SMM-25) with ArrayIt quill type ChipMaker pins (CMP3).
• Add 10l 3xSSC 1.5M betaine to the dried PCR product.
• Spot volumes range from .1 to .9 nl. While spot diameters are approximately 100m, normally ranging from 90 to 110m.
• Spot distance is 180m center to center.

Post-print processing

1. Leave slides in a dust free environment over night.
2. Transfer slides to metal slide racks for baking at 80C for 1.5 hours.
3. Prepare 0.2% SDS solution and pour 350ml into each slide container. This will cover the slides sufficiently.
4. Dip slide holders repeatedly into the SDS solution and allow to soak for 2 minutes on shaker.
5. Transfer to millipore water for 2 minutes. Repeat water wash one additional time.
6. Soak slides in boiling water for 2 minutes. without agitation.
7. Dip in 95% ethanol and spin dry at 500 rpm for 1 minutes.
8. Store slide in slide box with dessicant at room temperature

Library Descriptions

• CT9993 Library
• IR62266 Libraries
• IRRI Library
• Nippon-Bare Libraries
• Pokkali Libraries

Gal Files

• 031003 Rice
• 031003 Rice 48 blocks
• 042803 Rice 32 blocks
• Rice 070303