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The following are large animations, for best viewing save them to your computer.
RNAi Line 9 Water Loss (22.4 MB)
RNAi Line 9 Water Recovery (10.6 MB)
Methods:
Plants (2 weeks old) were grown on sealed 0.5 x MS agar (3% sucrose) plates. For the observation of water loss, the lid was removed and plants were exposed to open air. Pictures were taken in 1 min intervals for 2 hours. Following desiccation, the plate was sealed again and the plants began to recover. Pictures were taken in two-minute intervals for one hour during the recovery process. Animation was generated via Animation Shop 3, based on these pictures.
10 T2 PIP1b RNAi plants from 5 lines, together with two wild type plants, were used in this experiment. Below is a picture to show the lines (2 plants/line) derived from independent primary transformants.
Stay tuned for more movies and complete confirmation of the RNAi effect by RT-PCR.
