Biology 100 and 101
University of
Illinois

Integrative Biology 100
Discussion Class,
April
7-9

#11 DNA Analysis - DNA Fingerprinting and DNA Sequencing


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Related Assignment:

Learner Objectives

You should be able to:

  1. Describe the action of restriction enzymes in recombinant DNA technology and fingerprinting techniques.

  2. Explain the effects of mutation and inheritance of DNA that provide the theoretical basis for the use of DNA profiling, also called DNA fingerprinting, as a tool for the identification of individuals or determination of the relationships of organisms.

  3. Explain the effects of mutation and inheritance of DNA that provide the theoretical basis for the use of DNA fingerprinting, also called DNA profiling, as a tool for the identification of individuals or determination of the relationships of organisms.

  4. Interpret simulated DNA evidence and decide on the guilt or innocence of the suspect.

  5. Briefly describe how a segment of DNA is sequenced using the Sanger, or cycle sequencing, method.

Web Resources for this Discussion

DNA Interactive

Collection of Biotechnology Animations from Cold Spring Harbor
The animations dealing with PCR , Southern Blotting, and Cycle Sequencing are especialy good.

Sequence for Yourself from The Nova Program: Cracking the Code of Life. This animation is rather detailed, but you have been exposed to all the basic concepts you need to understand how genes are sequenced.

    This is another possibility for extra credit. Watch the entire program and write it up as a Media Watcher" extra credit project.

Some Applications of DNA Fingerprinting:

  • DNA Detective from The Dolan DNA Learning Center, Cold Spring Harbor Laboratory.

      Animation of DNA fingerprinting and summaries of several real examples solved using DNA fingerprint techniques

  • Electrophoresis animation from DNA Interactive.

    • Select "Techniques at the bottom of the screen
      Select "Sorting and Sequencing" from the top of the next screen.
      Select the "Gel Electrophoresis" animation.

  • New Methods of DNA Profiling using PCR and STRs (Short Tandem Repeats). DNA Interactive: Applications

    • Select "Human Identification from the bottom of the first screen

    • Select "profiling" from the top of the second screen.

    • Roll your mouse over the right hand circle near the top of the screen ("Today's DNA Profile") and click to select the animation.

The Sanger Method of DNA Sequencing, AKA "Cycle Sequencing"